Registration Dossier
Registration Dossier
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 215-676-4 | CAS number: 1341-49-7
Long-term toxicity to fish
Administrative data
Link to relevant study record(s)
Description of key information
No data are available for AMBI. A 21-day study in Oncoryhychus mykiss with sodium fluoride is reviewed in the EU RAR and fluoride ICD (RIVM), giving an LC5 value of 5 mg/L. Data for ammonia report very low values for non-ionised ammonia (NH3), however these are not relevant as AMBI will dissociate to NH4 under environmental conditions with very little non-ionised ammonia present.
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 4 mg/L
Additional information
No data are available for the substance, ammonium hydrogendifluoride (AMBI). The substance is water-soluble and, in aqueous environments, will dissociate to form hydrogen, fluoride and ammonium ions. Read-across data are available from the soluble fluoride salt, sodium fluoride and from ammonia/ammomium salts.
Fluoride data
In a 21 day test with Oncorhynchus mykiss, an LC5 value of 4 mg/L was reported for sodium fluoride (actual concentration). This value is considered to be equivalent to the NOEC for mortality. The test was conducted in very soft (12 mg CaCO3/L) natural water with daily renewal of the test water.
Ammonia data
Pink salmon (Oncorhynchus gorbuscha) alevins were exposed to concentrations of unionized ammonia ranging between 0 and 4 mg/L (Rice & Bailey, 1980). The highest exposure concentration of ammonia caused significant decreases in weight of exposed fry in all three exposure groups. At 2.4 mg/L un-ionized ammonia, the groups held for 40 days and 61 days were significantly smaller in length and weight and at 1.2 mg/L un-ionized ammonia there was no significant difference. The NOEC was therefore concluded to be 1.2 mg/L un-ionised NH3. Effects were consistently more adverse for groups held 61 days. Juvenile channel catfish (L. punctatus) were exposed to 12 ammonia concentrations ranging from 48-2048mg un-ionized NH3-N/L for 31 days (Colt & Tchobanoglous, 1978). On a wet weight basis, growth was reduced by 50% at 517 ug/L un-ionized NH3-N and no growth occurred at 967 ug/L un-ionized NH3-N and higher. Above 500 ug/L un-ionized NH3-N, there was increasing damage to the dorsal and pectoral fins. The overall NOEC for the growth and weight was < 48 ug un-ionized NH3-N/L. Fertilised rainbow trout eggs were exposed to 0.05, 0.10, 0.19, 0.28, and 0.37 mg NH3-N/L for periods of either 25 or 33 days followed by 42 days post hatch observations. Egg mortality was not affected in either run by any of the ammonia concentrations. Growth and development of rainbow trout sac fry are inhibited by long-term exposures to concentrations of ammonia as low as 0.05 mg NH3-N/L (Burkalter & Kaya, 1977).
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
