Ammonium hydrogendifluoride

Repeated dose toxicity: inhalation

Administrative data

Endpoint:

short-term repeated dose toxicity: inhalation

Type of information:

migrated information: read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Older, non-GLP published study.

Cross-reference

Reason / purpose for cross-reference:

reference to same study

Data source

Reference

Reference Type:

publication

Title:

Age sensitivity of the body to fluoride ions in the air.

Author:

Sadilova MS et al

Year:

1974

Bibliographic source:

Gig. Sanit. 1974; 12:33-36

Materials and methods

Test guideline

Qualifier:

no guideline followed

Principles of method if other than guideline:

Repeated exposure inhalation toxicity in rats

GLP compliance:

no

Remarks:

: older published study, pre-dates GLP

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

Hydrogen fluoride

EC Number:

231-634-8

EC Name:

Hydrogen fluoride

Cas Number:

7664-39-3

IUPAC Name:

fluoride

Details on test material:

No further details

Test animals

Species:

rat

Strain:

not specified

Sex:

female

Details on test animals or test system and environmental conditions:

Female rats of various ages were used in this test. No environmental conditions are given.

Administration / exposure

Route of administration:

inhalation: vapour

Type of inhalation exposure:

whole body

Vehicle:

other: unchanged (no vehicle)

Details on inhalation exposure:

Exposure of female rats of various age to 1 mg HF/m3 for 1 month, 6 hours per day.

Analytical verification of doses or concentrations:

not specified

Details on analytical verification of doses or concentrations:

There was no analytical determination of concentration. The concentration stated is nominal.

Duration of treatment / exposure:

1 month

Frequency of treatment:

6 hours per day.

Doses / concentrations

Remarks:

Doses / Concentrations:
1 mg/m3
Basis:
nominal conc.

No. of animals per sex per dose:

The number of animals is not stated.

Control animals:

not specified

Details on study design:

Exposure of female rats of various age to 1 mg HF/m3 for 1 month, 6 hours per day.

Examinations

Sacrifice and pathology:

Examination of the lungs,airways, bones and teeth at the end of the exposure.

Other examinations:

No further examination

Statistics:

None

Results and discussion

Results of examinations

Clinical signs:

effects observed, treatment-related

Mortality:

mortality observed, treatment-related

Body weight and weight changes:

not examined

Food consumption and compound intake (if feeding study):

not examined

Food efficiency:

not examined

Water consumption and compound intake (if drinking water study):

not examined

Ophthalmological findings:

not examined

Haematological findings:

not examined

Clinical biochemistry findings:

not examined

Urinalysis findings:

not examined

Behaviour (functional findings):

not examined

Organ weight findings including organ / body weight ratios:

not examined

Gross pathological findings:

effects observed, treatment-related

Histopathological findings: non-neoplastic:

effects observed, treatment-related

Details on results:

Exposure of female rats of various age to 1 mg HF/m3 for 1 months, 6 hr/d resulted in damage to the dental enamel. This effect was especially seen in young animals and in animals of 17.6 to 18.6 months of age. The respiratory organs of the young animals showed atrophy and local oedema of the bronchial mucosa. In the older animals, the lungs showed peribronchial hyperplasia. In animals of about 12 months the formation of irregular shaped cavities in their bones

Effect levels

Dose descriptor:

LOAEC

Effect level:

1 mg/m³ air

Sex:

female

Basis for effect level:

other: Effects on the teeth, bones and respiratory tract were seen at the single concentration of HF investigated in this study.

Target system / organ toxicity

Critical effects observed:

not specified

Any other information on results incl. tables

See above

Applicant's summary and conclusion

Conclusions:

Exposure to HF vapour for one month resulted in effects on the teeth, bones and respiratory tract.

Executive summary:

Exposure of female rats of various ages to 1 mg HF/m3 for 1 months, 6 hr/d resulted in damage to the dental enamel. This effect was most marked in young animals and in animals of 17.6-18.6 months of age. The respiratory organs of the young animals showed atrophy and local oedema of the bronchial mucosa. In older animals, the lungs showed peribronchiqal hyperplasia. In animals of about 12 months old the formation of irregular shaped cavities in bones was noted.