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EC number: 700-840-4
CAS number: 160583-22-2
OS-REp models treated with the test substance and the controls could be
included into the data analysis process. The
the relative viabilities of the OS-REp models topically treated with 2
-phenylethyl cyanoacrylate and
the controls. The test substance did not significantly decrease the
relative tissue viability. With 97.3% the mean value is similar to that
of the negative control. According to the prediction model 2
-phenylethyl cyanoacrylate can be regarded as non-irritating to the skin.
relative viability data of the OS-REp models after topical application
of the indicated substances (mean ± SD). Viability has been determined
after 35 minutes substance exposure time and 42 hours of
post-incubation. All data are calculated compared to the negative
control (= 100% rel. viability)
Mean rel. viability
- The the mean optical
density of the negative control was 1,214 +/- 0,103 absorption units.
- The positive control
(5% SDS solution) killed the tissue models nearly completely, resulting
in a remaining activity of 1.33 +/- 0.47%
2 -Phenylethyl cyanoacrylate (PhECA) was tested for skin irritation on a
human three dimensional epidermal model (OS-REp, Henkel). PhECA were
applied topically with the help of a 25 µl spoon to the skin model,
moistened with PBS and applied for 35 minutes before the substance was
washed away from the models. Application was followed by a 42 hours
incubation period, then the cytotoxic (irritancy) effect was determined.
Cytotoxicity is expressed as the reduction of mitochondrial
dehydrogenase activity measured by formazan production from MTT at the
end of the treatment. Skin irritation is expressed as the remaining cell
viability after exposure to the test substance. The relative mean tissue
viability obtained after treatment with PhECA was 85% compared to the
negative control tissues. Since the mean relative tissue viability for
PhECA was above 50% it is considered to be non irritant. The positive
control (SDS) had a mean cell viability of about 1 %. The absolute mean
OD570 (optical density at 570 nm) of the negative control tissues was
1.2. The standard deviation value of the percentage viability of three
tissues treated identically was less than 18%, indicating that the test
system functioned properly.
Finally, it is concluded that this test is valid and that PhECA is
non-irritant in the in vitro skin irritation test under the experimental
conditions described in this report.
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