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Toxicological information

Skin irritation / corrosion

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Administrative data

Endpoint:
skin irritation: in vivo
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: Well performed OECD and GLP guideline study.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1989
Report Date:
1989

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 404 (Acute Dermal Irritation / Corrosion)
Version / remarks:
of 1981
Deviations:
no
GLP compliance:
yes

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report): Chloranil

Test animals

Species:
rabbit
Strain:
New Zealand White
Details on test animals and environmental conditions:
New Zealand White rabbits conventional breeding, with appropriate range of bodyweight (2.0 to 2.7 kg).
Room temperature: 20°+- 3°C
Humidity (%): 50+-20.
Commercially available rabbit diet and hay were fed. Deionized, chlorinated water was fed ad libitum.

Test system

Type of coverage:
semiocclusive
Preparation of test site:
shaved
Vehicle:
other: polyethylene glycol 400
Controls:
not required
Amount / concentration applied:
0.5 g test substance mixed with 0.2 ml polyethylene glycol 400.
Duration of treatment / exposure:
4 hours (remnant test substance was removed by rinsing with lukewarm water)
Observation period:
Examinations at 30-60 min, 24, 48, and 72h after patch removal.
Number of animals:
3
Details on study design:
Only animals with intact skin were used. Control animals were not required, as untreated skin areas of the test animal can serve as control. After the 4-hour exposure period residual test substance was cautiously removed from the exposed skin. The treated skin patches were evaluated at: 0.5-1 hour post end of exposure and at 24, 48 and 72 h post end of exposure.

Results and discussion

In vivo

Resultsopen allclose all
Irritation parameter:
erythema score
Basis:
other: animal # 1, 2, 3 each
Time point:
other: mean of 24, 48, 72 h
Score:
0
Reversibility:
other: initial, slight response in a single animal was fully reversible within 24h
Irritation parameter:
edema score
Basis:
other: animal 1, 2, 3 each
Time point:
other: mean of 24, 48,72 h
Score:
0
Reversibility:
other: no signs of irritation at any time
Other effects:
No other effects were reported.

Any other information on results incl. tables

Gradings of Skin Reactions Attained for each Animal at Different Observation Time Points

Time after Patch Removal

0.5 – 1 h

24 h

48 h

72 h

Animal (Identification) No.

1  2  3

1  2  3

1  2  3

1  2  3

Erythema

0  1  0

0  0  0

0  0  0

0  0  0

Edema

0  0  0

0  0  0

0  0  0

0  0  0

Applicant's summary and conclusion

Interpretation of results:
not irritating
Remarks:
Migrated information Criteria used for interpretation of results: expert judgment
Conclusions:
The mean Draize scores for erythema and edema derived from 24, 48 and 72 hour post treatment observation time points in 3 rabbits were 0.0 each. The only erythema finding (very slight in degree) seen in this study in one animal at 0.5 to 1 hour post patch removal was completely reversible within 24 hours post patch removal. Based on these finding the test substance is neither skin irritating nor skin corrosing.
Executive summary:

2,3,5,6-tetrachloro-1,4-benzoquinone was tested for skin irritation according to OECD Guideline 404 and in compliance with GLP. Reliability grade 1 was assigned to the study. Each of three New Zealand White rabbits was treated for 4 hours by semi-occlusive administration of a single dose of 0.5 g test substance moistened with polyethylene glycol 400 to shaved intact skin (approximately 6 cm2/animal). The site of administration was examined for skin reactions at 0.5-1, 24, 48 and 72 hours post patch removal. The only finding during this study was an erythema very slight in degree (Grade 1, barely perceptible) in one animal at 0.5 -1 hour post patch removal. This finding was completely reversible within 24 hours post patch removal. No edema reaction was evident during the entire study. Mean Draize scores for erythema and edema derived from the latter three observation time points were 0.0 each. Based on these finding the test substance is neither skin irritating nor skin corrosing.