Registration Dossier

Administrative data

Endpoint:
activated sludge respiration inhibition testing
Type of information:
experimental study
Adequacy of study:
supporting study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Basic data given: comparable to actual guideline/ standards; test procedure according to national standards
Cross-reference
Reason / purpose:
reference to same study

Data source

Referenceopen allclose all

Reference Type:
study report
Title:
Unnamed
Year:
1989
Report Date:
1989
Reference Type:
review article or handbook
Title:
BUA Report 85, Ref. Hoechst, 1989e
Author:
Beratergremium für umweltrelevante Altstoffe der Gesellschaft Deutscher Chemiker (BUA)
Year:
1992
Bibliographic source:
Hirzel Verlag, Stuttgart

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to
Guideline:
EU Method C.11 (Biodegradation: Activated Sludge Respiration Inhibition Test)
Deviations:
not specified
Qualifier:
according to
Guideline:
OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
Deviations:
not specified
GLP compliance:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent

Sampling and analysis

Analytical monitoring:
no

Test solutions

Vehicle:
no

Test organisms

Test organisms (species):
activated sludge

Study design

Test type:
not specified
Water media type:
freshwater
Limit test:
no
Total exposure duration:
3 h

Results and discussion

Effect concentrationsopen allclose all
Duration:
3 h
Dose descriptor:
EC50
Effect conc.:
455.5 mg/L
Nominal / measured:
nominal
Conc. based on:
not specified
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate
Duration:
3 h
Dose descriptor:
other: EC20
Effect conc.:
135 mg/L
Nominal / measured:
nominal
Conc. based on:
not specified
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate
Duration:
3 h
Dose descriptor:
other: EC80
Effect conc.:
> 1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
not specified
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate

Applicant's summary and conclusion

Validity criteria fulfilled:
not specified
Conclusions:
The 3h-EC50 was 455.5 mg/L, the 3h-EC20 was 135 mg/L.
Executive summary:

In a 3-h toxicity study activated sludge from a domestic water treatment plant was exposed toChloranil (2,3,5,6-tetrachloro-1,4-benzoquinone)in accordance with EU Method C.11 and OECD Guideline 209. The study was not performed in compliance with GLP. Reliability grade 2 was assigned. Tested concentrations and existence of controls were not indicated. The 3h-EC20 was 135 mg/L. The 3h-EC50 was 455.5 mg/L.