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Toxicological information

Acute Toxicity: oral

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Administrative data

Endpoint:
acute toxicity: oral
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Acceptable, well documented study report performed before OECD and GLP guideline implementation. Important aspects in line with current OECD guidelines.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1975
Report Date:
1975

Materials and methods

Test guideline
Qualifier:
equivalent or similar to
Guideline:
OECD Guideline 401 (Acute Oral Toxicity)
Deviations:
not specified
GLP compliance:
no
Remarks:
prior to GLP implementation
Test type:
standard acute method
Limit test:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report): Chloranil (2,3,5,6-tetrachlorbenzochinon-1,4)

Test animals

Species:
rat
Strain:
Wistar
Sex:
female
Details on test animals and environmental conditions:
Female rats were used, as no sex linked differences in sensitivity were seen in preliminary tests. Body weight at treatment = 90 - 132 g, average weight = 107 g. Commercially available rat diet and tap water were fed ad libitum, except during the fasting period prior to and after administration, when food was withdrawn from 16 hours before until 2 hours post administration.

Administration / exposure

Route of administration:
oral: gavage
Vehicle:
other: sesame oil
Details on oral exposure:
The test substance was suspended in the vehicle, sesame oil. The test substance was administered as a 25% prepartaion in the vehicle.
Doses:
2500, 4000, 6300, 10000, 15000 mg/kg bw
No. of animals per sex per dose:
10 females
Control animals:
no
Details on study design:
Post-dosing observation period was 14 days during which mortality was recorded. Body weight was recorded at study start (day of dosing) for each animal and 7 and 14 days after application for each survivor. Premature deaths and survivors killed at the end of the 14-day post-dosing observation period were necropsied and macroscopic findings recorded.
Statistics:
Probit analysis

Results and discussion

Effect levels
Sex:
female
Dose descriptor:
LD50
Effect level:
6 951 mg/kg bw
Based on:
test mat.
Remarks on result:
other: Only females tested, as there were no indications of sex linked differences in sensitivity in pretests.
Mortality:
2500 mg/kg: 0/10 (f)
4000 mg/kg: 0/10 (f)
6300 mg/kg: 4/10 (f)
10000 mg/kg: 9/10 (f)
15000 mg/kg: 10/10 (f)

Mortality occurred within 1/2 to 8/9 days post treatment.
Clinical signs:
The general condition of decedent animals became increasingly worse prior to death.
Body weight:
2500 mg/kg bw. Initially / after 7 days / after 14 days: 107 / 125 / 148
4000 mg/kg bw. Initially / after 7 days / after 14 days: 99 / 116 / 143
6300 mg/kg bw. Initially / after 7 days / after 14 days: 125 / 132 / 164
10000 mg/kg bw. Initially / after 7 days / after 14 days: 103 / 89 / 112
15000 mg/kg bw. Initially / after 7 days / after 14 days: 102 / 92 / ---
Gross pathology:
Necropsy of dead and finally killed animals did not reveal any overt signs of toxicity.

Applicant's summary and conclusion

Interpretation of results:
not classified
Remarks:
Migrated information Criteria used for interpretation of results: expert judgment
Conclusions:
The oral LD50 in female rats was found to be 6951 mg/kg bw.
Executive summary:

2,3,5,6-tetrachloro-1,4-benzoquinone was tested for its acute toxicity by oral gavage administration in female rats. Only female rats were included in this study, as there was no indication of sex linked differences in sensitivity in preliminary tests. An LD50 of 6951 mg/kg bw was calculated. At a single dose of 4000 mg/kg and at all lower doses administered, all animals survived the 14-day post treatment observation period and body weights were not adversely affected. The higher doses of 6300, 10000 and 15000 mg/kg induced a dose-related increase in mortality (40%, 90% and 100%, respectively) with transient bodyweight loss in one female survivor each at 6300 mg/kg and at 10000 mg/kg, and body weight loss over 7 days post treatment followed by premature death in one female treated with 15000 mg/kg. In all other survivors of this study bodyweight was not adversely affected by treatment. The general condition of decedent animals became increasingly worse prior to death. Necropsy did not reveal any overt signs of toxicity in premature deaths or animals which survived until the scheduled necropsy day.