Registration Dossier

Administrative data

Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
June 08 - August 03, 1979
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Comparable to guideline study but no GLP

Data source

Referenceopen allclose all

Reference Type:
study report
Title:
Unnamed
Year:
1979
Reference Type:
review article or handbook
Title:
BUA Report 85, Ref. Hoechst, 1979
Author:
Beratergremium für umweltrelevante Altstoffe der Gesellschaft Deutscher Chemiker (BUA)
Year:
1992
Bibliographic source:
Hirzel Verlag, Stuttgart

Materials and methods

Test guideline
Qualifier:
equivalent or similar to
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
GLP compliance:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent

Sampling and analysis

Analytical monitoring:
no

Test solutions

Vehicle:
no

Test organisms

Test organisms (species):
Leuciscus idus melanotus

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h

Results and discussion

Effect concentrationsopen allclose all
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
4.6 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Duration:
96 h
Dose descriptor:
NOEC
Effect conc.:
1.6 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
behaviour
Duration:
96 h
Dose descriptor:
LC0
Effect conc.:
2.5 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Duration:
96 h
Dose descriptor:
LC100
Effect conc.:
6.3 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality

Any other information on results incl. tables

Symptoms: several toxic effects (apathy, accelerated respiration, swimming along the water surface, supine position, and anesthesia) were observed at test concentrations above 1.6 mg/L. At 6.3 mg/L all fish died within the first 48 hours.

Applicant's summary and conclusion

Validity criteria fulfilled:
not applicable
Remarks:
The following validity criteria from OECD 203 are fulfilled: the mortality in the control did not exceed 10% at the end of the test; the dissolved oxygen concentration was at least 60% of the air saturation value throughout the test.
Conclusions:
The 96h-LC50 determined in this study was 4.6 mg/L.
Executive summary:

In a 96-h acute toxicity study, golden orfe (Leuciscus idus melanotus) were exposed to 3-Hydroxy-2-naphthoic acid at nominal concentrations of 0 (control), Chloranil (2,3,5,6-tetrachloro-1,4-benzoquinone)at nominal concentrations of 0 (control), 1.6, 2.5, 4.0, and 6.3 mg/L under static conditions. No guideline was followed but the principle of the method was equivalent to OECD guideline 203. The study was not performed in compliance with GLP. Reliability grade 2 was assigned.The 96-h LC50 was 4.6 mg/L.Several toxic effects (apathy, accelerated respiration, swimming along the water surface, supine position, and anesthesia) were observed at test concentrations above 1.6 mg/L. At 6.3 mg/L all fish died within the first 48 hours.