Solid Recovered Fuel (Municipal Solid Waste and similar wastes, processed)

Acute Toxicity: dermal

Administrative data

Endpoint:

acute toxicity: dermal

Type of information:

experimental study

Adequacy of study:

key study

Study period:

21 July, 2011

Reliability:

1 (reliable without restriction)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2011

Report date:

2011

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 402 (Acute Dermal Toxicity)

GLP compliance:

yes

Test type:

standard acute method

Limit test:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Solid Recovered Fuel (Municipal Solid Waste and similar wastes, processed)

EC Number:

700-869-2

Molecular formula:

Not applicable

IUPAC Name:

Solid Recovered Fuel (Municipal Solid Waste and similar wastes, processed)

Test material form:

solid: particulate/powder

Remarks:

migrated information: powder

Test animals

Species:

rat

Strain:

Crj: CD(SD)

Sex:

male/female

Administration / exposure

Type of coverage:

occlusive

Vehicle:

unchanged (no vehicle)

Details on dermal exposure:

Skin preparation:
24 hours before the beginning of the test the back and the sides of the animals have been shaved on an area of about 20 cm2.
Application:
The test sample has been given, a ratio of 2000 mg/kg, with a square of gauze directly on animals' skin humidified with sodium chloride injection and fixed with a skin patch in a area of about 10% of the total body surface.
the square has been put on the back of animals and further fixed with hypo-allergenic occlusive adhesive tape.
The whole trunk of the animal has been protected with an elastic bandage. Bandage has been left in situ for 24 hours, during this period animals have been kept in single cages.
Removal:
24 hours after application bandage and adhesive tape have been remove.
Skin has been cleaned from sample excess by a tampon soaked in physiological solution.

Duration of exposure:

24 hours

Doses:

The test sample has been given, a ratio of 2000 mg/kg, with a square of gauze directly on animals' skin humidified with sodium chloride injection and fixed with a skin patch in a area of about 10% of the total body surface.

No. of animals per sex per dose:

10 (5 males and 5 females)

Control animals:

not specified

Details on study design:

Observations
The general status of all animals has been daily monitored after 1-3-5 hours from the start of treatment and daily (5 days per week) for 14 days.
All activies related to the study, as well as remarks and exams have been daily registered in dated and signed forms.
During the study the following observations have been done:
- Mortality
Animals have been observed in the morning of each working day (5 days/week).
- Clinical symptomatology
Every clinical symptom, including possible variations of somato-motory activity, was daily registered for each individual animal.
Clincial observations included a general objective exam (G.O.E.) above:
1.assessment of major organic functions;
2. assessment of the status of integumentary apparatus;
3. assessment of the status of mucosae;
4. assessment of somato-motory activity and of the sensory status.
- Body weight
Animals have been weighted before experiment, after 7 days and at the end of the experiment.
- Post mortem
At the end of the observation period, animals have been sacrificed and a post-mortem examination has been carried out.

Results and discussion

Effect levels

Sex:

male/female

Dose descriptor:

LD50

Effect level:

> 2 000 mg/kg bw

Based on:

test mat.

Mortality:

During the study no mortality was detected.

Clinical signs:

other: During the study neither symptoms nor signs of toxicity were recorded.

Other findings:

- Post mortem observations
At the autopsy no abnormalities have been detected.

Applicant's summary and conclusion

Interpretation of results:

GHS criteria not met

Conclusions:

The test substance has a LD50 > 2000 mg/kg bw and can be considered NOT TOXIC - NOT HARMUFUL.