Solid Recovered Fuel (Municipal Solid Waste and similar wastes, processed)

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

From August 30 to September 3, 2011

Reliability:

1 (reliable without restriction)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2011

Report date:

2011

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Test guideline 2

Qualifier:

according to guideline

Guideline:

other: EN 14735:2005 Characterization of waste-preparation of waste sample for ecotoxicity tests.

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Solid Recovered Fuel (Municipal Solid Waste and similar wastes, processed)

EC Number:

700-869-2

Molecular formula:

Not applicable

IUPAC Name:

Solid Recovered Fuel (Municipal Solid Waste and similar wastes, processed)

Test material form:

solid: particulate/powder

Remarks:

migrated information: powder

Details on test material:

Product: CarboNeXT
Physical state: solid
Batch: 08-07-2011

Test organisms

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

14 fishes have been used, 7 of them treated with the extract of the test substance, the other 7 fishes were used as control.

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Test conditions

Hardness:

245 mg CaCO3/L

Test temperature:

23+/-2°C

pH:

pH has remained within required limits (6.0 - 8.5).

Nominal and measured concentrations:

100 mg/l

Details on test conditions:

Medication: Animals, 7 for group, have been added to the assay tanks with a biomass density lower than 1 g/L.
Exposition: The treated group and the control group have been observed for 96 hours with a photoperiod of 12 hours of light and 12 hours of darkness.

Results and discussion

Effect concentrations

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Any other information on results incl. tables

Sublethal observations / clinical signs:

Mortality

No case of mortality has been detected in treated animals and in control ones:

Symptomatology

No toxic symptom has been detected in treated animals and in control ones:

% of oxygen saturation

The percentage of saturation has remained for the whole length of the assay above 60% both in control and in treated.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The obtained results, in compliance with assay validity criteria, shows that n dead fishes at the concentration of 100 mg/L of the extract of the test substance after 96 hours were observed; the LC50 value after 96 hours results higher than 100 mg/L of the extract of the test substance.