Registration Dossier

Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
experimental study
Adequacy of study:
key study
Study period:
From September 5 to September 9, 2011
Reliability:
1 (reliable without restriction)

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2011
Report Date:
2011

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to
Guideline:
OECD Guideline 201 (Alga, Growth Inhibition Test)
Qualifier:
according to
Guideline:
other: EN 14735:20058 Characterization of waste-preparation of waste sample for ecotoxicity tests
Qualifier:
according to
Guideline:
other: EN 12457-2:2002 - Characterisation of waste - Leaching - Compliance test for leaching of granular waste materials and sluges; Part 2: One stage batch test at a liquid to solid ratio of 10 L/kg for materials with particle size below 4 mm
GLP compliance:
yes

Test material

Reference
Name:
Unnamed
Type:
Constituent
Test material form:
solid: particulate/powder
Remarks:
migrated information: powder
Details on test material:
Product: CarboNeXT
Physical state: solid
Batch: 08-07-2011

Test organisms

Test organisms (species):
Pseudokirchneriella subcapitata (previous names: Raphidocelis subcapitata, Selenastrum capricornutum)
Details on test organisms:
culture medium : sterile 250 ml Erlenmeyer flasks
temperature: 23 +/- 2 °C
lightning: greater than 4300 lux
stirring: steady with electrical stirrer

Study design

Test type:
not specified
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
72 h

Test conditions

Test temperature:
23 +/- 2 °C
Details on test conditions:
starting cellular density: 10^4
no. of replications: 6
no.of concentrations: 1 (100 mg/l)
lightning: continuous uniform fluorescent lamp 6000 lux
stirring: orbital shaker (continuous)

Results and discussion

Effect concentrationsopen allclose all
Duration:
72 h
Dose descriptor:
other: Ir % (algae growth Inhibition)
Effect conc.:
4 other: %
Basis for effect:
growth rate
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
> 100 mg/L
Basis for effect:
growth rate

Any other information on results incl. tables

   Days  Result  Validity criteria
 Growth rate  0 -1  1.58  >0.92
   1 -2  1.38  
   2 -3  1.53  
 %CV for section by section  0 -1  9.44  <35%
   1 -2  10.56  
   2 -3  2.72  
 %CV whole period  0 -3  0.78  <7%

The cell concentration in the control culture are increased of a factor greater than 16 in three days that corresponds to a specific growth rate of 0.92 day^-1.

A normal and healthy appearance of the algae at the end of the test was observed at microscopic.

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
The obtained results, in compliance with assay validity criteria, showed that the Algae Growth inibitition (Ir %) after 72 hours and at the concentration of 100 mg/L of the extract of the test substance is equal to 4%.