Solid Recovered Fuel (Municipal Solid Waste and similar wastes, processed)

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

From September 5 to September 7, 2011

Reliability:

1 (reliable without restriction)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2011

Report date:

2011

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Test guideline 2

Qualifier:

according to guideline

Guideline:

other: EN 14735:2005 Characterization of waste-preparation of waste sample for ecotoxicity tests

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Solid Recovered Fuel (Municipal Solid Waste and similar wastes, processed)

EC Number:

700-869-2

Molecular formula:

Not applicable

IUPAC Name:

Solid Recovered Fuel (Municipal Solid Waste and similar wastes, processed)

Test material form:

solid: particulate/powder

Remarks:

migrated information: powder

Details on test material:

Product: CarboNeXT
Physical state: solid
Batch: 08-07-2011

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

Age: 6 - 24 hours
No of animals: 20 for test sample - 20 for control
No of group: 4

Study design

Test type:

not specified

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Post exposure observation period:

After 24 and 48 hours from the beginning of the test, the immobilisation of the Daphnie has been evaluated verifying if the organisms, after light stirring of the vessels, were not capable to move in the following 15 seconds.
pH, dissolved oxygen and temperature of the assay environment have been measured at the beginning and at the end of the test (after 48 hours).

Quality criteria
- The immobilisation of control animals must not be higher than 10% at the end of the test.
- The concentration of dissolved oxygen in the vessels used in the assay ought to be higher than 3 mg/L for during the assay. However, in any case, the concentration of dissolved oxygen must not go below 2 mg/L.
- pH must not change for more than 1.5 units.
- The final concentration of the test substance must be included in a range corresponding to +/-20°C of the initial value, to be able to attribute obtained results to nominal concentration. Otherwise they have to be attributed to the final measured concentration.

Test conditions

Hardness:

between 140 and 250 mg CaCO3/L

Test temperature:

21.2°C at beginning of the test and 21.0°C at the end of the test.

pH:

Approx. 7.55 at beginning of the test and approx. 8.46 at the end of the test.

Dissolved oxygen:

> 3 mg/L

Nominal and measured concentrations:

100 mg/l

Details on test conditions:

Since the test sample is constituted by different hardly identifiable analytically components, it has been decided not to evaluate the stability of the test substance during the test.

Results and discussion

Effect concentrations

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The obtained results, in compliance with test acceptability criteria, showed that no immobilised Daphnie were observed with a concentration of 100 mg/L of the extract of the test substance CarboNeXT after 48 hours. The values of EC50 can be considered higher than 100 mg/L of the extract of the test substance.