2-[[2-(dimethylamino)ethyl]methylamino]ethanol

Toxicity to microorganisms

Link to relevant study record(s)

Reference

Endpoint:

activated sludge respiration inhibition testing

Type of information:

experimental study

Adequacy of study:

key study

Study period:

Exposure: 26. February 1999

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Guideline study (GLP)

Qualifier:

according to guideline

Guideline:

OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test

Qualifier:

according to guideline

Guideline:

ISO 8192 (Water quality - Test for inhibition of oxygen consumption by activated sludge for carbonaceous and ammonium oxidation)

Qualifier:

according to guideline

Guideline:

other: EC guideline 88/302/EEC

GLP compliance:

yes

Specific details on test material used for the study:

Batch number Vers. 98/044-10

Analytical monitoring:

no

Vehicle:

no

Test organisms (species):

activated sludge, domestic

Details on inoculum:

- Source: Activated sludge from laboratory wastewater plants treating municipal and synthetic sewage.
- Preparation of inoculum for exposure: The inoculum was washed, brought to a concentration of 5 g/I dry substance and aerated for 24 hours .
50 ml were added to a total volume of 250 ml to obtain a concentration of 1 g/I dry substance in the test .

Test type:

static

Water media type:

freshwater

Total exposure duration:

30 min

Test temperature:

20 ±2°C

pH:

7.4

Nominal and measured concentrations:

Nominal test concentration: 1000 mg/L

Details on test conditions:

TEST CONCENTRATIONS
- Test concentrations: 1000 mg/L

TEST SYSTEM
- Test vessel: Erlenmeyer glass vessel (nominal value 250 mL)
- Fill volume: 250 mL
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 3

Reference substance (positive control):

yes

Remarks:

3,5-dichlorophenol

Duration:

30 min

Dose descriptor:

other: EC20

Effect conc.:

> 1 000 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

inhibition of total respiration

Remarks:

respiration rate

Results with reference substance (positive control):

EC50 of 3,5 -dichlorophenol in the range of 5-30 mg/I: yes

Reported statistics and error estimates:

Graphical interpreation of the results.

Summary of measurements:

Vessel no.:	BC 1	BC 2	BC 3	mvBC	TS 1
Concentration of test substance (mg/L)	-	-	-	-	1000
Added volume of stock solution (mL/vessel)	-	-	-	-	125.5
Direct addition (mg/vessel)	-	-	-	-	-
Oxygen concentration start (mg O2/L)	8.3	8.2	8.0	-	8.4
Oxygen concentration after 6 min (mg O2/L)	5.7	5.8	5.5	-	6.0
Oxygen consumption rate (mg O2/L*6 min)	2.6	2.4	2.5	2.5	2.4
Oxygen consumption rate (mg O2/L*h)	26	24	25	25	24
Specific oxygen consumption rate (mg O2/g*h)	26	24	25	25	24
Change in oxygen consumption rate compared with the blank control (%)	-	-	-	-	4

- BC: blank control

- mvBC: mean value of blank control

- TS: test substance

 

pH values – blank control (BC) and test substance (TS):

Vessel no.:	BC 1	BC 2	BC 3	TS 1
pH-value before adding the inoculum before correction: after correction:	6.7 7.2	6.7 7.2	6.7 7.3	9.5 7.4
pH-value after an incubation time of 30 min	-	-	-	7.6

Validity criteria fulfilled:

yes

Conclusions:

Based on the results of oxygen consumption, the EC50 (30 minutes) for 2-[[2-(Dimethylamino)ethyl]methylamino]ethanol [CAS# 2212-32-0]was estimated to be >1000 mg/L.

Executive summary:

The toxicity to bacteria of 2-[[2-(Dimethylamino)ethyl]methylamino]ethanol [CAS# 2212-32-0] (identified as Halb-PMDETA in the study report) was investigated according to OECD 209. Activated sludge (mixed population of different micro-organisms) was exposed for 30 minutes to a nominal concentration of 1000 mg/L. Two controls without test substance are included in the test design. 3,5-dichlorophenol was used as a reference substance.

Based on the results of oxygen consumption, the EC50 (30 minutes) for 2-[[2-(Dimethylamino)ethyl]methylamino]ethanol [CAS# 2212-32-0] was estimated to be >1000 mg/L, indicating low toxicity of 2-[[2-(Dimethylamino)ethyl]methylamino]ethanol [CAS# 2212-32-0] to aerobic waste water treatment bacteria.

Description of key information

The EC50 (30 minutes) for 2-[[2-(Dimethylamino)ethyl]methylamino]ethanol [CAS# 2212-32-0]was estimated to be >1000 mg/L

Key value for chemical safety assessment

EC50 for microorganisms:

1 000 mg/L

Additional information

The toxicity to bacteria of 2-[[2-(Dimethylamino)ethyl]methylamino]ethanol [CAS# 2212-32-0] (identified as Halb-PMDETA in the study report) was investigated according to OECD 209. Activated sludge (mixed population of different micro-organisms) was exposed for 30 minutes to a nominal concentration of 1000mg/L. Two controls without test substance are included in the test design. 3,5-dichlorophenol was used as a reference substance.

Based on the results of oxygen consumption, the EC50 (30 minutes) for 2-[[2-(Dimethylamino)ethyl]methylamino]ethanol [CAS# 2212-32-0]was estimated to be >1000 mg/L, indicating low toxicity of 2-[[2-(Dimethylamino)ethyl]methylamino]ethanol [CAS# 2212-32-0]to aerobic waste water bacteria.