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EC number: 603-837-5
CAS number: 134605-64-4
Key study: BBA Part VI, 1-1, SETAC, No significant effects on respiration or nitrification, Grade 1997.
The toxicity of the test material to soil microorganisms was determined
in a soil respiration and nitrification study performed to GLP and in
line with the standardised guidelines BBA Part VI, 1-1 and SETAC. The
microbial biomass of the soil -loamy sand -Collombey- was determined
prior to application of the test material by measuring substrate-induced
respiration to confirm adequate microbial activity. On the basis of this
biomass determination, the possible effects of the test material on soil
respiration were evaluated after the addition of 1000 mg glucose/100 g
dry soil equivalents during the constant initial respiration phase i.e.
1 to 6 hours after glucose amendment. Nitrogen conversion were
determined by measuring the content of NH4-N,
NO3-N and NO2-N.
All post-application measurements were performed in Lucerne amended
soil, calibrated with the reference substance dinoseb and related to
untreated control. The test material was applied to the soil as acetone
solution. The initial nominal test concentrations in soil were 0.67 and
1.67 mg test material per kg soil dry weight corresponding to
approximately two- and five-fold the proposed field application rate
(i.e. 500 and 1250 g a.i./ha).
Non significant test material induced effects on soil short-term
respiration rates were observed. Short-term respiration rates recorded
during the constant initial respiration phase in soil treated with two-
and five-fold the maximum proposed field application rate deviate by <6
and <5% over the whole period from the untreated control, respectively.
In the loamy sand, total-N concentrations at the end of the incubation
period, i.e. after 28 days, deviated by <17% and <9% from the control at
two- and five-fold application rate, respectively. These figures
indicate that the test material has no long lasting effects on
It can be concluded that the incorporation of the test material in the
tested soil showed no effect on short-term respiration rates with the
two-fold application rate. Even when applied with the five-fold
application rate, the test material showed no effects. N-mineralisation
was not affected in the loamy sand. Therefore, it can be concluded that
the test material showed no long lasting effects on N-mineralisation of
the tested agricultural soil. No adverse effect of the test material on
organic matter turnover, and hence on soil fertility, is to be expected
from its use.
The study was performed to a high standard, in line with GLP and
standardised guidelines. Accordingly the study has been assigned a
reliability score of 1 in line with the principles for assessing data
quality set out in Klimisch (1997). The available data are deemed to be
relevant, reliable and adequate for the purposes of risk assessment.
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