Oligomerisation and alkylation reaction products of 2-phenylpropene and phenol

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

04 Jun. - 12 Aug. 2008

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Principles of method if other than guideline:

open test system

GLP compliance:

yes (incl. QA statement)

Analytical monitoring:

yes

Details on sampling:

Samples for TOC-analysis were taken at 0 h and 48 h

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Water Accommodated Fractions (WAFs) prepared by stirring various amounts of the test item in Daphnia-medium
for 48 h with magnetic stirrers (open conditions). The WAFs were prepared in 2-L glass beakers.
The beakers were equipped with a glass tube that allowed the separation of the water phase by siphoning.
The test loading rates of the TS were weighed in weighing scoops that afterwards were placed into the glass beakers.
The beakers were then filled with Daphnia-medium.
Mixing was carried out at a speed that was slow enough not to cause dispersion or emulsification of the undissolved fraction
of the test item. To ensure this, the vortex developed at the surface by stirring was set at ~ 10 % of the water depth.
After stirring for 48 h the WAFs were allowed to stand for 1 h before use to facilitate phase separation.
The extracts gained with this method were clear.

- Pre-conditioning: The test vessels of the Definite Daphnia-Test were rinsed with control media and WAFs for ca. 0.5 h before the definite
start of the tests to facilitate adsorption of the test item to the glass walls of the beakers.
Control media and WAFs were then discarded, and the test vessels were refilled again with control media and WAFs, respectively.

- Differential loading: control, 1, 10 and 100 mg/L (range finding)
control, 5 mg/L, 10 mg/L, 25 mg/L, 50 mg/L, and 100 mg/L
- Controls: without test material (blank; Daphnia medium)
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): none
- Evidence of undissolved material (e.g. precipitate, surface film, etc): no

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Daphnia
- Strain: --
- Source: origin German Environmental Ministry
- Age at study initiation (mean and range, SD): =< 24 h
- Method of breeding: The animals are cultivated in a climate-controlled room (light/dark = 14 h/10 h; illumination: Gro-Lux fluorescent tubes).

- Feeding during test
- Food type: The animals are fed with algae (Desmodesmus subspicatus)

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Post exposure observation period:

none

Hardness:

see data under "salinity"

Test temperature:

20.6 – 21.0 °C (20 °C ± 2 °C)

pH:

7.8 - 8.2 at 0, 24 and 48 h

Dissolved oxygen:

8.0 - 8.2 mg/L at 0, 24 and 48 h

Salinity:

The total of Ca- and Mg-ions was 2.5 mmol/L, the ratio of Ca/Mg was 4:1, the ratio of Na/K was 10:1.
The total alkalinity of the solution was 0.8 mmol/L, the pH-value was 7.8 ± 0.2.

Nominal and measured concentrations:

Main test series:
Time [h] Control 5 mg/L 10 mg/L 25 mg/L 50 mg/L 100 mg/L (loading)
==================================================================
0 0.3 1.4 n.d. 2.9 n.d. 7.1 mg C/L (TOC of WAF)
48 1.5 1.8 n.d 3.5 n.d. 8.1 mg C/L (TOC of WAF)
===================================================================

Range finding:
Time [h] Control 1 mg/L 10 mg/L 100 mg/L (Loading)
=======================================================
0 0.3 0.5 1.6 6.6 mg C/L (TOC of WAF)
48 0.3 0.8 1.3 6.3 mg C/L (TOC of WAF)
=========================================================
Note: TOC values include background values.

Details on test conditions:

TEST SYSTEM
- Test vessel:
- Type (delete if not applicable): open
- Material, size, headspace, fill volume: glass beaker, 50 mL, test volume: 20 mL
- Aeration: none
- Renewal rate of test solution (frequency/flow rate): none
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- No. of vessels per vehicle control (replicates): not applicable


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: reconstituted
- Total organic carbon: no data
- Alkalinity: 0.8 mmol/L
- Ca/Mg ratio: 4:1
- Conductivity: no data
- Culture medium different from test medium: no


OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16 h light, 8 h dark
- Light intensity: no data, Gro-Lux fluorescent tubes


TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2 - 2.5
- Range finding study
- Test concentrations: 100 and 1000 mg/L (loading)
- Results used to determine the conditions for the definitive study:
immobilisation 100 %

Reference substance (positive control):

no

Key result

Duration:

48 h

Dose descriptor:

EL50

Remarks:

effective loading

Effect conc.:

14 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Remarks:

Novares LA 100

Basis for effect:

mobility

Remarks on result:

other: determination of EL50 by Probit-analysis

Duration:

48 h

Dose descriptor:

EL10

Remarks:

effective loading

Effect conc.:

6 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Remarks:

Novares LA 100

Basis for effect:

mobility

Duration:

48 h

Dose descriptor:

NOELR

Remarks:

No-observable effect loading rate

Effect conc.:

5 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Remarks:

Novares LA 100

Basis for effect:

mobility

Remarks:

1/20 immobile

Duration:

48 h

Dose descriptor:

LOELR

Remarks:

Lowest observable effect loading rate

Effect conc.:

10 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Remarks:

Novares LA 100

Basis for effect:

mobility

Remarks:

6/20 immobile

Details on results:

- Other biological observations: suppression of moulting at >= 25 mg/L
- Mortality/immobilisation in control: 1/20
- Other adverse effects control: none
- Abnormal responses: no data
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: none
- Effect concentrations exceeding solubility of substance in test medium: none

Reported statistics and error estimates:

Determination of the EL50 (EL= Effective Loading) and NOELR was accomplished using Probit-analysis (Finney-method, lognormal distribution; confidence limit 95 %, significance level: p <0.05).

Table 1: Immobilisation rate in dependence of time and loading rate, number of carapace moults, and

number of living animals trapped at the surface [number per total animals, %] (Report Tab. 8)

Control

5 mg/L

10 mg/L

25 mg/L

50 mg/L

100 mg/L

Number

[%]

Number

[%]

Number

[%]

Number

[%]

Number

[%]

Number

[%]

Immobilisation 24 h

0/20

0

0/20

0

3/20

15

6/20

30

17/20

85

20/20

100

Immobilisation 48 h

1/20

5

1/20

5

6/20

30

14/20

70

20/20

100

20/20

100

Moults 48 h

14/20

70

13/20

65

14/20

70

6/20

30

2/20

10

0/20

0

Living animals at the surface 48 h

0/20

0

0/20

0

0/20

0

0/20

0

0/20

0

0/20

0

Validity criteria fulfilled:

yes

Remarks:

- Immobilisation in the control: 5 % (nominal value ≤10 %) - Dissolved oxygen in all vessels: >= 8.0 mg/L (nominal value >= 3 mg/L)

Reference 2

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

10 Aug. - 16 Aug. 2010

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Principles of method if other than guideline:

open test system

GLP compliance:

yes (incl. QA statement)

Specific details on test material used for the study:

- Name of test material (as cited in study report): Novares LA 300
- Lot/batch No.: 28166
- Composition of test material: composition is specified in IUCLID Sect. 13 - Assessment reports under Certificate of Analysis_Novares LA 300_phenol, methylstyrenated
- Stability under test conditions: no measured data; based on chemical structure assumed to be stable
- Storage condition of test material: room temperature, exclusion of light

Analytical monitoring:

yes

Details on sampling:

Samples for TOC-analysis were taken at 0 h and 48 h

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Water Accommodated Fractions (WAFs) prepared by stirring various amounts of the test item in Daphnia-medium
for 24 h with magnetic stirrers (open conditions). The WAFs were prepared in 2-L glass beakers.
The beakers were equipped with a glass tube that allowed the separation of the water phase by siphoning.
The test loading rates of the TS were weighed in weighing scoops that afterwards were placed into the glass beakers.
The beakers were then filled with Daphnia-medium.
Mixing was carried out at a speed that was slow enough not to cause dispersion or emulsification of the undissolved fraction
of the test item. To ensure this, the vortex developed at the surface by stirring was set at ~ 10 % of the water depth.
After stirring for 24 h the WAFs were allowed to stand for 1 h before use to facilitate phase separation.
The extracts gained with this method were clear.

- Pre-conditioning: The test vessels of the Definite Daphnia-Test were rinsed with control media and WAFs for ca. 0.5 h before the definite
start of the tests to facilitate adsorption of the test item to the glass walls of the beakers.
Control media and WAFs were then discarded, and the test vessels were refilled again with control media and WAFs, respectively.

- Differential loading: control, 2.5, mg/L, 5.0 mg/L, 10 mg/L, 25 mg/L, and 50 mg/L
- Controls: without test material (blank; Daphnia medium)
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): none
- Evidence of undissolved material (e.g. precipitate, surface film, etc): no

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Daphnia
- Strain: --
- Source: origin German Environmental Ministry
- Age at study initiation (mean and range, SD): =< 24 h
- Method of breeding: The animals are cultivated in a climate-controlled room (light/dark = 14 h/10 h; illumination: Gro-Lux fluorescent tubes).

- Feeding during test
- Food type: The animals are fed with algae (Desmodesmus subspicatus)

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Post exposure observation period:

none

Hardness:

see data under "salinity"

Test temperature:

20.8 – 21.5 °C (20 °C ± 2 °C)

pH:

7.8 - 8.0 at 0, 24 and 48 h

Dissolved oxygen:

7.8 - 8.1 mg/L at 0, 24 and 48 h

Salinity:

The total of Ca- and Mg-ions was 2.5 mmol/L, the ratio of Ca/Mg was 4:1, the ratio of Na/K was 10:1.
The total alkalinity of the solution was 0.8 mmol/L, the pH-value was 7.8 ± 0.2.

Nominal and measured concentrations:

Main test series:
Time [h] Control 2.5 mg/L 10 mg/L 50 mg/L (loading)
=================================================
0 0.4 1.4 1.2 2.9 mg C/L (TOC of WAF)
48 0.4 0.8 1.1 2.8 mg C/L (TOC of WAF)
=================================================
Note: TOC values include background values. / n.d. = not determined

Details on test conditions:

TEST SYSTEM
- Test vessel:
- Type (delete if not applicable): open
- Material, size, headspace, fill volume: glass beaker, 50 mL, test volume: 20 mL
- Aeration: none
- Renewal rate of test solution (frequency/flow rate): none
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- No. of vessels per vehicle control (replicates): not applicable


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: reconstituted
- Total organic carbon: no data
- Alkalinity: 0.8 mmol/L
- Ca/Mg ratio: 4:1
- Conductivity: no data
- Culture medium different from test medium: no


OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16 h light, 8 h dark
- Light intensity: no data, Gro-Lux fluorescent tubes


TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2 - 2.5
- Range finding study: none, results of a previous fish test with LA 300 served as guide.

Reference substance (positive control):

no

Key result

Duration:

48 h

Dose descriptor:

EL50

Remarks:

effective loading

Effect conc.:

17 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Remarks:

Novares LA 300

Basis for effect:

mobility

Remarks on result:

other: determination of EL50 by Probit-analysis

Duration:

48 h

Dose descriptor:

NOELR

Remarks:

No-observable effect loading rate

Effect conc.:

10 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Remarks:

Novares LA 300

Basis for effect:

mobility

Remarks:

0/20 immobile

Duration:

48 h

Dose descriptor:

LOELR

Remarks:

Lowest observable effect loading rate

Effect conc.:

25 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Remarks:

Novares LA 300

Basis for effect:

mobility

Remarks:

19/20 immobile

Details on results:

- Other biological observations: suppression of moulting at 50 mg/L
- Mortality/immobilisation in control: 0/20
- Other adverse effects control: none
- Abnormal responses: no data
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: none
- Effect concentrations exceeding solubility of substance in test medium: none

Reported statistics and error estimates:

Determination of the EL50 (EL= Effective Loading) and NOELR was accomplished using Probit-analysis (Finney-method,
lognormal distribution; confidence limit 95 %, significance level: p <0.05).

Table 1: Immobilisation rate in dependence of time and loading rate, number of carapace moults, and

number of living animals trapped at the surface [number per total animals, %] (Report Tab. 3)

Control

2.5 mg/L

5.0 mg/L

10 mg/L

25 mg/L

50 mg/L

Number

[%]

Number

[%]

Number

[%]

Number

[%]

Number

[%]

Number

[%]

Immobilisation 24 h

0/20

0

0/20

0

0/20

0

0/20

0

10/20

50

12/20

60

Immobilisation 48 h

0/20

0

0/20

0

0/20

0

0/20

0

19/20

95

20/20

100

Moults 48 h

19/20

99

18/20

90

20/20

100

20/20

100

17/20

85

13/20

65

Living animals at the surface 48 h

0/20

0

0/20

0

0/20

0

0/20

0

0/20

0

0/20

0

Validity criteria fulfilled:

yes

Remarks:

- Immobilisation in the control: 0 % (nominal value ≤10 %) - Dissolved oxygen in all vessels: >= 7.8 mg/L (nominal value >= 3 mg/L)

Reference 3

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

10 Jun. - 10 Oct. 2008

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Principles of method if other than guideline:

open test system

GLP compliance:

yes (incl. QA statement)

Specific details on test material used for the study:

- Name of test material (as cited in study report): Novares LA 700
- Lot/batch No.: 23861
- Composition of test material: composition is specified in IUCLID Sect. 13 - Assessment reports under Certificate of Analysis_Novares LA 700_phenol, methylstyrenated
- Stability under test conditions: no measured data; based on chemical structure assumed to be stable
- Storage condition of test material: room temperature, exclusion of light

Analytical monitoring:

yes

Details on sampling:

Samples for TOC-analysis were taken at 0 h and 48 h
centrifugation at 3950 rpm for 10 minutes

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Water Accommodated Fractions (WAFs) prepared by stirring various amounts of the test item in Daphnia-medium
for 24 h with magnetic stirrers (open conditions). The WAFs were prepared in 2-L glass beakers.
The beakers were equipped with a glass tube that allowed the separation of the water phase by siphoning.
The test loading rates of the TS were weighed in weighing scoops that afterwards were placed into the glass beakers.
The beakers were then filled with Daphnia-medium.
Mixing was carried out at a speed that was slow enough not to cause dispersion or emulsification of the undissolved fraction
of the test item. To ensure this, the vortex developed at the surface by stirring was set at ~ 10 % of the water depth.
After stirring for 24 h the WAFs were allowed to stand for 1 h before use to facilitate phase separation.
The extracts gained with this method were clear.

- Pre-conditioning: The test vessels of the Definite Daphnia-Test were rinsed with control media and WAFs for ca. 0.5 h before the definite
start of the tests to facilitate adsorption of the test item to the glass walls of the beakers.
Control media and WAFs were then discarded, and the test vessels were refilled again with control media and WAFs, respectively.

- Differential loading: control, 1 mg/L, 10 mg/L , 100 centrifugation (range finding)
control, 5 mg/L, 10 mg/L, 25 mg/L, 50 mg/L, and 100 mg/L
- Controls: without test material (blank; Daphnia medium)
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): none
- Evidence of undissolved material (e.g. precipitate, surface film, etc): no

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Daphnia
- Strain: --
- Source: origin German Environmental Ministry
- Age at study initiation (mean and range, SD): =< 24 h
- Method of breeding: The animals are cultivated in a climate-controlled room (light/dark = 14 h/10 h; illumination: Gro-Lux fluorescent tubes).

- Feeding during test
- Food type: The animals are fed with algae (Desmodesmus subspicatus)

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Post exposure observation period:

none

Hardness:

see data under "salinity"

Test temperature:

20.8 – 21.5 °C (20 °C ± 2 °C)

pH:

8.0 - 8.2 at 0, 24 and 48 h

Dissolved oxygen:

7.6 - 8.0 mg/L at 0, 24 and 48 h

Salinity:

The total of Ca- and Mg-ions was 2.5 mmol/L, the ratio of Ca/Mg was 4:1, the ratio of Na/K was 10:1.
The total alkalinity of the solution was 0.8 mmol/L, the pH-value was 7.8 ± 0.2.

Nominal and measured concentrations:

Main test series:
Time [h] Control 5 mg/L 10 mg/L 25 mg/L 50 mg/L 100 mg/L 250 mg/L (loading)
==================================================================
0 0.3 0.7 n.d. n.d 1.3 n.d. 5.1 mg C/L (TOC of WAF)
48 0.1 0.4 n.d n.d. 1.2 n.d. 4.7 mg C/L (TOC of WAF)
===================================================================

Range finding:
Time [h] Control 1 mg/L 10 mg/L 100 mg/L (loading)
=======================================================
0 0.3 0.4 0.7 1.3 mg C/L (TOC of WAF)
48 0.5 1.2 1.2 1.4 mg C/L (TOC of WAF)
=========================================================
Note: TOC values include background values. / n.d. = not determined

Details on test conditions:

TEST SYSTEM
- Test vessel:
- Type (delete if not applicable): open
- Material, size, headspace, fill volume: glass beaker, 50 mL, test volume: 20 mL
- Aeration: none
- Renewal rate of test solution (frequency/flow rate): none
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- No. of vessels per vehicle control (replicates): not applicable


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: reconstituted
- Total organic carbon: no data
- Alkalinity: 0.8 mmol/L
- Ca/Mg ratio: 4:1
- Conductivity: no data
- Culture medium different from test medium: no


OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: 16 h light, 8 h dark
- Light intensity: no data, Gro-Lux fluorescent tubes


TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2 - 2.5
- Range finding study
- Test concentrations: 100 and 1000 mg/L (loading)
- Results used to determine the conditions for the definitive study:
immobilisation 100 %

Reference substance (positive control):

no

Key result

Duration:

48 h

Dose descriptor:

EL50

Remarks:

effective loading

Effect conc.:

51 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Remarks:

Novares LA 700

Basis for effect:

mobility

Remarks on result:

other: determination of EL50 by Probit-analysis

Duration:

48 h

Dose descriptor:

EL10

Remarks:

effective loading

Effect conc.:

20 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Remarks:

Novares LA 700

Basis for effect:

mobility

Duration:

48 h

Dose descriptor:

NOELR

Remarks:

No-observable effect loading rate

Effect conc.:

10 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Remarks:

Novares LA 700

Basis for effect:

mobility

Remarks:

0/20 immobile

Duration:

48 h

Dose descriptor:

LOELR

Remarks:

Lowest observable effect loading rate

Effect conc.:

25 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Remarks:

Novares LA 700

Basis for effect:

mobility

Remarks:

3/20 immobile

Details on results:

- Other biological observations: suppression of moulting at 250 mg/L
- Mortality/immobilisation in control: 0/20
- Other adverse effects control: none
- Abnormal responses: no data
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: none
- Effect concentrations exceeding solubility of substance in test medium: none

Reported statistics and error estimates:

Determination of the EL50 (EL= Effective Loading) and NOELR was accomplished using Probit-analysis (Finney-method, lognormal distribution; confidence limit 95 %, significance level: p <0.05).

Table 1: Immobilisation rate in dependence of time and loading rate, number of carapace moults, and

number of living animals trapped at the surface [number per total animals, %] (Report Tab. 8)

Control

5 mg/L

10 mg/L

25 mg/L

50 mg/L

100 mg/L

250 mg/L

Number

[%]

Number

[%]

Number

[%]

Number

[%]

Number

[%]

Number

[%]

Number

[%]

Immobilisation 24 h

0/20

0

0/20

0

0/20

0

0/20

0

6/20

30

2/20

10

19/20

95

Immobilisation 48 h

0/20

0

0/20

0

0/20

0

3/20

15

13/20

65

14/20

70

20/20

100

Moults 48 h

18/20

90

19/20

95

18/20

90

17/20

85

15/20

75

19/20

95

1/20

5

Living animals at the surface 48 h

0/20

0

0/20

0

0/20

0

0/20

0

0/20

0

0/20

0

0/20

0

Validity criteria fulfilled:

yes

Remarks:

- Immobilisation in the control: 0 % (nominal value ≤10 %) - Dissolved oxygen in all vessels: >= 7.6 mg/L (nominal value >= 3 mg/L)

Description of key information

Due to substance properties, short-term toxicity to aquatic invertebrates is determined based on loadings. The lowest EL50 value of three tests using three technical products of 'Oligomerisation and alkylation reaction products of 2-phenylpropene and phenol' is selected to characterise the acute toxicity to daphnia.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

14 mg/L

Additional information

The substance 'Oligomerisation and alkylation reaction products of 2-phenylpropene and phenol' (OAPP) (previous name phenol, methylstyrenated) is a UVCB substance with a complex and within limits variable composition. Water solubility is low and not clearly defined due to the differing solubility of individual components. Depending on the quantity used, concentration of material dissolved in saturated water samples is variable. Therefore, water accommodated fractions (WAFs) have been used in aquatic toxicity tests. Thus, results are presented as loadings (LL/EL50).

Toxicity to Daphnia has been tested in valid GLP guideline studies (OECD TG 202) with three different technical products of OAPP (LA 100, LA 300, and LA 700). All three products are composed of the same constituents and differ only in the quantitative composition of components. They are subsumed under the same EC Number (700-960-7).

The solubility of the three technical products is somewhat different. Therefore, it can be expected that aqueous effect levels differ to some degree. For reasons of comprehensive protection, the lowest EL50 value (14 mg/L) (LA 100) is selected as key value for the chemical safety assessment.