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EC number: 701-325-7 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in mammalian cells
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- migrated information: read-across based on grouping of substances (category approach)
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Research publication. Well documented meets generally accepted scientific principles, acceptable for assessment. Read across to the registered substance is considered scientifically justified.
Data source
Reference
- Reference Type:
- publication
- Title:
- Genotoxicity of iron compounds in Salmonella typhimurium and L5178Y mouse lymphoma cells.
- Author:
- Dunkel V C, San R H C, Seifried H E, Whittaker P
- Year:
- 1 999
- Bibliographic source:
- Environ Mol Mutagenesis 33: 28-41
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 476 (In Vitro Mammalian Cell Gene Mutation Test)
- Principles of method if other than guideline:
- Method: other: Clive et al/1975 and 1979
- GLP compliance:
- not specified
- Type of assay:
- mammalian cell gene mutation assay
Test material
- Reference substance name:
- Iron trichloride
- EC Number:
- 231-729-4
- EC Name:
- Iron trichloride
- Cas Number:
- 7705-08-0
- Molecular formula:
- Cl3Fe
- IUPAC Name:
- Iron (III) trichloride
- Details on test material:
- The substance tested was the hexahydrate (CAS number 10025-77-1), but this does not affect the chemical species available to the test organisms.
Constituent 1
Method
Species / strain
- Species / strain / cell type:
- mouse lymphoma L5178Y cells
- Metabolic activation:
- with and without
- Metabolic activation system:
- Aroclor induced rat liver S9
- Test concentrations with justification for top dose:
- without S9 309-1030, with S9 0.206-1.236
- Vehicle / solvent:
- Vehicle used: distilled water
Controlsopen allclose all
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- Remarks:
- distilled water
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- other: methylmethanesulphonate
- Remarks:
- without activation
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- 9,10-dimethylbenzanthracene
- Remarks:
- with activation
- Details on test system and experimental conditions:
- METHOD OF APPLICATION: in medium; in agar (plate incorporation); preincubation; in suspension; as impregnation on paper disk
DURATION
- Preincubation period: 4 hours
- Exposure duration: 4 hours
- Expression time (cells in growth medium): 48 hours
- Selection time (if incubation with a selection agent): 10-12 days
- Fixation time (start of exposure up to fixation or harvest of cells): 12-14 days
SELECTION AGENT (mutation assays): trifluorothymidine
NUMBER OF REPLICATIONS: duplicate cultures
NUMBER OF CELLS EVALUATED: 1E+06 for mutant selection, 200/plate for mutant count, colony size range 0.2-1.1 mm.
DETERMINATION OF CYTOTOXICITY
- Method: relative total growth - Evaluation criteria:
- Doubling of mutant frequency over concurrent solvent treated control value together with dose relationship.
- Statistics:
- Colonies larger than 0.1 mm diameter were counted with an Artek automated colony counter. Colony size was also determined.
Results and discussion
Test results
- Species / strain:
- mouse lymphoma L5178Y cells
- Metabolic activation:
- with and without
- Genotoxicity:
- positive
- Remarks:
- without activation
- Cytotoxicity / choice of top concentrations:
- other: without S9 ca 1030 µg Fe/ml; with S9 >1.236 µg Fe/ml
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not applicable
- Positive controls validity:
- valid
- Remarks on result:
- other: other: L5178Y TK+/- 3.7.C mouse lymphoma cells
- Remarks:
- Migrated from field 'Test system'.
Any other information on results incl. tables
Table 1: Number of revertants per plate (mean of 2 plates)
Concentration µg Fe/ml |
Mutant frequency* |
Growth** |
Concentration µg Fe/ml |
Mutant frequency* |
Growth** |
-MA |
- MA |
+MA |
+MA |
||
0* |
26 |
100 |
0 |
44 |
100 |
309.0 |
28 |
49.5 |
0.206 |
45 |
115..5 |
412 |
24 |
50.5 |
0.412 |
50 |
94.5 |
515 |
28 |
32.5 |
0.824 |
98 |
85.5 |
618 |
32 |
35 |
1.030 |
107 |
76.5 |
1030 |
49 |
12.5 |
1.236 |
121 |
66 |
Positive control |
450 |
49 |
Positive control |
447 |
16 |
* per 1E+06 survivors
** as % of control
PRECIPITATION CONCENTRATION: None reported
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
negative with metabolic activation
In the mouse lymphoma TK+/- assay ferric chloride FeCl3.6H2O showed a negative response in the absence of metabolic activation and a positive dose related response in the presence of metabolic activation.
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