Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 205-381-9
CAS number: 139-89-9
The corneas treated with HEDTA-Na3 ‘as it is’ showed opacity values
ranging from -1 to 7 and permeability values ranging from 2.214 to
4.998. The corneas were slightly hazy after the 240 minutes of treatment
with HEDTA-Na3 ‘as it is’. Although on one cornea a part of the cornea
was clear. No pH effect of the test substance was observed on the
rinsing medium. Hence, the in vitro irritancy scores ranged from 37 to
82 after 240 minutes of treatment with HEDTA-Na3 ‘as it is’.
Table 2. Summary of opacity, permeability, and in vitro scores.
MeanIn vitroIrritation Score1, 2
HEDTA-Na3 ‘as it is’
using the negative control mean opacity and mean permeability values.
vitro irritancy score (IVIS) = mean opacity value + (15 x mean OD490value).
Screening for the eye irritancy potential of HEDTA-Na3 has been
undertaken using the Bovine Corneal Opacity and Permeability test (BCOP
test) based on the most recent OECD and EC test guidelines. The possible
ocular irritancy of undiluted HEDTA-Na3 (as is) were tested through
topical application for approximately 240 minutes.
Undiluted HEDTA-Na3 (as is) induced severe ocular irritation
mainly through the permeability endpoint, resulting in a mean in
vitro irritancy score of 59 after 240 minutes of treatment. Since
undiluted HEDTA-Na3 induced an IVIS≥55.1, it is concluded that HEDTA-Na3
tested ‘as is’ is corrosive or severe irritant in the Bovine Corneal
Opacity and Permeability test and
should be labelled (GSH) as Category 1 according to the criteria of
Regulation (EC) No. 1272/2008.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
Tällä verkkosivustolla käytetään evästeitä parhaan mahdollisen käyttäjäkokemuksen varmistamiseksi.
Welcome to the ECHA website. This site is not fully supported in Internet Explorer 7 (and earlier versions). Please upgrade your Internet Explorer to a newer version.
Do not show this message again