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EC number: 205-381-9
CAS number: 139-89-9
Daphnia magna were exposed to the test substance under static conditions
at 17 degrees C for 48 hours and the NOEC, LC50, as well as partial kill
and 100% kill was assessed. LC Values were calculated using Thompson's
method of moving averages. The LC50 was 192 mg/L, indicating relatively
low toxicity to daphnids. There was limited test condition data
reported. Analytical confirmation of the test substance was not
Table 1. Cumulative percent mortalities.
Versenol/120 Chel was of low toxicity to Acartia tonsa, only 40%
mortality was recorded at the highest test concentration of 2296 mg/L
after 48 hours. The extrapolated 48 hour LC50 was 2585 mg/L as
determined by the Maximum Likelihood-Probit method.
Daphnia magna were exposed to the test substance - Trilon D- for 48
hours and observed for mobility/mortality. There were no effects seen at
the highest concentration tested, 500 mg/L.
In this short-term test, Ca was present in overstoichiometric amounts. Thus, in the test system the Ca-complex was formed.
EC50(24h) calculated (confidence interval 95 %):----------------------------------------------------EDTA 610 mg/l (570-640)Mn(II)+EDTA 940 mg/l (800-1000)Fe(III)+EDTA 17 mg/l (13-21)Cu(II)+EDTA 38 mg/l (31-45)Zn(II)+EDTA 910 mg/l (840-980)Hg(II)+EDTA 0.00032 mg/l (0.00022-0.00052)Cd(II)+EDTA 310 mg/l (290-330)----------------------------------------------------All the EC50 values, except those obtained for the free EDTA, are expressed as mg/L of total amount of the studied metal. To compare the toxicities of the metal chelates with those of the free EDTA, all the EC50 values were converted to mg/L EDTA.
EC 50 values with 95% confidence limits were calculated by a probit analysis computer program, version 2.3 (National Swedish Environmental Protection Agency).The EC50 values of Fe3+, Cu2+, Mn2+, Zn2+, Cd2+ and Hg2+ complexed by EDTA were compared with those of the respective uncomplexed metals and free EDTA.The experiments gave the following toxicity order: Hg2+, Cu2+, Cd2+, Zn2+, Fe3+, Mn2+ (higher to lower toxicity).After complexation, the toxicities were 1.1-1700 (EDTA) times lower. With Hg2+ complexation did not decrease the toxicity. Compared with the toxicity of free EDTA, metal complexation increased the toxicity of EDTA.
number of studies have investigated the short-term toxicity to aquatic
invertebrates of trisodium HEDTA and the structural analogues disodium
EDTA and tetrasodium EDTA. The 24/48 h EC50 values range from 192 mg
Versenol acid/L in Daphnia magna to >2296 mg Na3-HEDTA/L in the
marine copepod Acartia tonsa.The lowest value for Na3-HEDTA of
192 mg Versenol acid/L in Daphnia magna was considered for the
CSA. Although water hardness is not given, this is the range of other
data obtained in fish for water of moderate hardness, which could be
seen as more representative of a typical environmental situation than
soft water. A study in a marine species exists but this was not used for
the CSA as no data on water hardness is available in the study and there
is not enough information to conclude to lower toxicity for marine
versus freshwater species.
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