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EC number: 270-302-7
CAS number: 68424-43-1
A study was performed to assess the effect
of the test item on the growth of the green
alga Desmodesmus subspicatus. The method
followed that described in the OECD
Guidelines for Testing of Chemicals (2006)
No 201, "Freshwater Alga and Cyanobacteria,
Growth Inhibition Test" referenced as Method
C.3 of Commission Regulation (EC) 440/2008.
Following a preliminary range-finding test,
Desmodesmus subspicatus was exposed to a
Water Accommodated Fraction (WAF) of the
test item, at a single nominal loading rate
of 100 mg/l (six replicate flasks) for 72
hours, under constant illumination and
shaking at a temperature of 24 ± 1 °C.
Samples of the algal
populations were removed daily and cell
concentrations determined for each control
and treatment group, using a Coulter®
Multisizer Particle Counter.
Exposure of Desmodesmus subspicatus to the
test item gave EL*50 values of greater than
100 mg/l loading rate WAF and
correspondingly the No Observed Effect
Loading Rate was 100 mg/l loading rate WAF.
It was considered unnecessary
and unrealistic to test at loading rates in
excess of 100 mg/l.
Total Organic Carbon (TOC)
analysis of the test preparations was
performed at 0 and 72 hours. Given the
background level of carbon in the control
vessels and also the low level of carbon in
the test vessels, it was considered that the
results gave no evidence of the presence of
test item in the WAF.
Therefore, given that the
toxicity cannot be attributed to a single
component or a mixture of components but to
the test item as a whole, and the dissolved
test item was below the quantifiable limit
of the analytical method, the results were
based on nominal loading rates only.
* EL =Effective Loading Rate
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