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EC number: 267-956-0
CAS number: 67953-76-8
Table 1. Summary of findings.
Result expressed as nominal concentration. Properties of the
test substance and evidence from other studies (where
concentrations were measured) indicate that nominal and
measured concentrations are likely to be in good agreement.
LC50 corrected for density (1.1) and % active acid (9.42%),
24 hr LC50: 2455 mg/L active
acid and 48 hr LC50: 2240 mg/L active acid (corrected),
uncorrected values were 24 hr LC50: 23644, 48 hr LC50: 21573 and 96 hr
LC50: 20997 all in uL/L (reported as ppm).
Table 1. Recorded mortalities.
Determined LC50 (other than the ones
24 h: 310 mg/L active acid
48 h: 270 mg/L active acid
day 4-6: 200 mg/L active acid
day 7 -14: 180 mg/L active acid
Table 2. Nominal and measured
concentrations of D2010 as active acid.
* author's note: Day 1 phosphorous values
were much lower than expected and were not used in the calculations for
mean Dequest 2010 levels. It was not determined why these samples did
not correspond to phosphorous concentrations measured previously (day 0)
and later (day 5). However, it was observed that sample aliquots during
the analytical process were cloudy; indicating an interfering
complex or incomplete sample digestion. An acceptable day 1 high
concentration indicated that no malfunctions had occurred in the test
In order to achieve D2010 from P values the
following equation was followed:
Total P (mg/L) * 5.54 (conversion factor for
converting P to 2010 ) * 0.601 (correction factor for percent purity) =
D2010 (mg/L active acid)
(96 h) LC50 195 mg/L (r-a) O. mykiss (ABC, 1979).
(96 h) LC50 2180 mg/L (r-a) C. variegatus (EG&G, 1976).
No data on short term toxicity of HEDP-xK salts, CAS 67593-76-8, to
freshwater or marine water fish are available.
The key studies have been
read-across to HEDP potassium salts from HEDP-H. The resulting LC50
once read across, taking into account ionisation state and MW, will be
higher for the phosphonic sodium and potassium salts than for the acid,
thereby confirming the low short-term toxicity to fish of these
The results of tests conducted
on HEDP and its salts are directly comparable, because the ionisation
state will depend only on the pH of the test medium. The counterion is
not considered to contribute to toxicity to fish. At
environmentally-relevant pH values, HEDP will be ionised typically three
times, and will form stable complexes with metal ions.
The lowest result in terms of
HEDP active acid is for a study with HEDP-H, therefore this study has
been chosen as key. A reliable 96 h LC50 value of 195 mg/L
(as active acid) has been determined for the effects of HEDP-H (CAS
2809-21-4) on mortality of Salmo gairdneri (now known as O.
mykiss) (ABC, 1979). This value is taken from a 14 day study, where
the 96 h LC50 has been reported. The same study determined a
14 d NOEC of 60 mg active acid/L and a 14 d LC50 of 180 mg
Other studies are available
indicating low short-term toxicity to fish with effects ranging between 200
to about 3000 mg/L for fish.
A reliable 96 h LC50
value of 2180 mg/L (as active acid) has been determined for the effects
of HEDP acid (CAS 2809-21-4) on mortality of Cyprinodon
variegatus (EG&G, 1976).
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