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Short-term toxicity to fish

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Description of key information

According to Guidance document Chapter R.7b, section R.7.8 short-term toxicity tests to fish do not need to be conducted if there are mitigating factors indicating that aquatic toxicity is unlikely to occur.

Nevertheless, the short-term toxicity to fish was calculated by EpiSuite's EcoSAR model. ECOSAR estimates the 96h LC50 for fish to be 375000 mg/L. This is far above water solubility so mortalities greater than 50% would not occur in a saturated solution during an exposure period of 96 hours.

Key value for chemical safety assessment

Additional information

According to Guidance document Chapter R.7b, section R.7.8 short-term toxicity tests to fish do not need to be conducted if there are mitigating factors indicating that aquatic toxicity is unlikely to occur.

Nevertheless, the short-term toxicity to fish was calculated by EpiSuite's EcoSAR model.

The suitability of the model was assessed and it was concluded that L-histidine is considered to be inside the applicability domain of the EpiSuite EcoSAR model.

ECOSAR estimates the 96h LC50 for fish to be 375000 mg/L.

This estimated LC50 value (375 g/L) exceeds the water solubility of L-histidine (43 g/L), therefore, it can be expected that mortalities greater than 50% would not occur in a saturated solution during an exposure period of 96 hours.

The QSAR results indicated that there is a very low toxicity to fish, and that the algae and invertebrates are more sensitive. For this reason, tests have been performed to investigate the toxicity towards algae and invertebrates. Taken together with the fact that in this way we can diminish the use of test animals by the use of alternative testing, which is one of the pillars of the REACH regulation, a short-term toxicity test for fish is not needed for L-histidine.