Registration Dossier

Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Guideline study with acceptable restrictions. No analytical dose verification was performed.

Data source

Referenceopen allclose all

Reference Type:
study report
Title:
Unnamed
Year:
1989
Report Date:
1990
Reference Type:
study report
Title:
Unnamed
Year:
2007
Report Date:
2007

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
other: DIN 38412 Part 9, 1982.
GLP compliance:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
Purity: no data

Sampling and analysis

Analytical monitoring:
no

Test solutions

Vehicle:
no

Test organisms

Test organisms (species):
Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h

Results and discussion

Effect concentrationsopen allclose all
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
244 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate
Duration:
72 h
Dose descriptor:
EC20
Effect conc.:
119 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate

Any other information on results incl. tables

Results of test BASF AG, 1989, 89/1320

During the 72 h exposure to Pentacarbonyliron fluorescence values were as follows (average of 4 replicates)*:

Nominal test fluorometer values after x hours
concentration
(mg/l) 0 24 48 72
-----------------------------------------------------------
Control 51 95 293 925
Solvent contr. 55 121 448 1954
3.9 52 143 533 1759
7.8 52 141 520 1701
15.625 50 131 510 1670
31.25 53 112 318 1188
62.5 49 110 302 1084
125 50 109 323 1079
250 48 85 133 563
500 48 29 15 19
-----------------------------------------------------------

*10000 cells/ml were introduced at the test start.


ECx values (in mg/l) after x hours of exposure

24 h 48 h 72 h
---------------------------------------------------------------------
EC20 188.1 28 19.1
EC50 357.5 190.2 149.5
EC90 - 435.5 418.3
---------------------------------------------------------------------

No chemical dose verification was performed. All values refer to nominal test concentrations of Pentacarbonyliron.
EC values are related to solubilizer control.

Remark: Strong growth support by solubilzer Chremophor RH 40 was observed.

---------------------------------------------------------------------


In a new mathematical evaluation, critical concentrations of BASF AG Algae test 89/1320, has been re-calculated, in order to allow an assessment based on growth rate and biomass (according to the requirements given in OECD guideline 201). (BASF AG 2007, 2/1320/89)

The mathematical evaluation was based on a linear relationship between fluorescence and cell number and raw data were equated with cell numbers.


Summary of Results for all Endpoints: Critical effect and threshold concentration as observed at end of experimental time; EC: Effective concentration for xx% reduction; LOEC: Lowest observed effect concentration; NOEC: No observed effect concentration

a) Yield
Critical conc.s
(mg/L) 0-24h 0-48h 0-72h
EC10 143.18 25.11 7.44
EC20 190.99 44.65 16.83
EC50 330.43 133.45 79.57
LOEC 31.25 31.25 <3.90
NOEC 15.60 15.60 <3.90

b) Growth rate
EC10 218.42 85.27 81.78
EC20 237.91 117.87 119.14
EC50 279.91 218.25 243.73
LOEC 250.00 31.25 31.25
NOEC 125.00 15.60 15.60

c) Sectional growth rate
EC10 218.42 85.44 30.04
EC20 237.91 113.31 76.55
EC50 279.91 193.88 453.69
LOEC 250.00 31.25 <3.90
NOEC 125.00 15.60 <3.90

d) Biomass integral
EC10 98.35 31.11 14.93
EC20 132.77 52.46 28.50
EC50 235.00 141.80 97.51
LOEC 31.25 31.25 15.60
NOEC 15.60 15.60 7.80

Remark: for 95% Confidence limits, please see BASF AG (2007), 2/1320/89

Applicant's summary and conclusion