Registration Dossier

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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

neurotoxicity: short-term oral
Type of information:
experimental study
Adequacy of study:
supporting study
2 (reliable with restrictions)
Reason / purpose for cross-reference:
reference to same study

Data source

Reference Type:
study report

Materials and methods

Test guideline
not specified
GLP compliance:
not specified
Limit test:

Test material

Constituent 1
Reference substance name:
Polyguard R (mixture of nonylphenol phosphites)
Polyguard R (mixture of nonylphenol phosphites)

Test animals

other: chicken
other: Hubbard

Administration / exposure

Route of administration:
oral: gavage
not specified
Analytical verification of doses or concentrations:
not specified
Frequency of treatment:
single exposure
Doses / concentrations
Doses / Concentrations:4000 mg/kg bwBasis:
No. of animals per sex per dose:
Control animals:
other: positive and negative control groups
Details on study design:
Type: other: delayed neurotoxicity

Results and discussion

Any other information on results incl. tables

Body weights were significantly reduced in the leptofos group (to 64%) and TOCP group (to 87%) with no effects in the Polygard group. Food intake was significantly higher in the Polygard groups in the second, third and fourth week, while leptofos induced a lower food intake. Animals in the leptofos and TOCP group showed signs of delayed neurotoxicity. Cholinesterase activity was not changed in any of the groups on all days. TOCP and leptofos showed degenerative changes in the axons and myelin sheath in the spinal cord and peripheral nerves. No degenerated axons were found in the control and Polygard group. Histological investigations of the other organs resulted in no effects caused by the different treatments.

Applicant's summary and conclusion

The authors concluded that it is not plausible that Polygard would result in delayed neurotoxicity in humans, based on the lack of clinical and electronmicroscopical detectable symptoms of delayed neurotoxicity after a single exposure to 4000 mg Polygard/kg bw to chickens.
Executive summary:

Polyguard R (mixture of nonylphenol phosphites) did not produce neurotoxicity in hens.