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Ecotoxicological information

Toxicity to microorganisms

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Administrative data

Endpoint:
toxicity to microorganisms, other
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2001
Reliability:
2 (reliable with restrictions)
Cross-reference
Reason / purpose for cross-reference:
reference to same study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2001
Report date:
2001

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
other: enhanced OECD 301D
Principles of method if other than guideline:
The study was an OECD 301D biodegradation study, though separate analysis was performed on the toxicity to the innoculum.
GLP compliance:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
4-(2-methyloctyl)phenyl bis(4-nonylphenyl) phosphite
EC Number:
701-028-2
Cas Number:
26523-78-4
Molecular formula:
C45H69O3P
IUPAC Name:
4-(2-methyloctyl)phenyl bis(4-nonylphenyl) phosphite
Constituent 2
Reference substance name:
Nonylphenol
EC Number:
246-672-0
EC Name:
Nonylphenol
Cas Number:
25154-52-3
IUPAC Name:
2-nonylphenol
Test material form:
liquid

Sampling and analysis

Analytical monitoring:
yes

Test solutions

Vehicle:
no
Details on test solutions:
15.4 mg/L was added to each replicate.

Test organisms

Test organisms (species):
other: Polyseed Commerical BOD innoculum
Details on inoculum:
Polyseed is a commerical bacterial inncolum encapsulated and used primarily for BOD studies.

Study design

Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
28 d

Test conditions

Test temperature:
20 +/- 1 deg C
Dissolved oxygen:
7.8% initial ; 3.0% final
Reference substance (positive control):
yes
Remarks:
sodium acetate

Results and discussion

Effect concentrations
Duration:
28 d
Dose descriptor:
EC0
Effect conc.:
15.4 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth inhibition
Details on results:
No inhibition of growth. Test concentration is well in excess of water solubility.

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
No inhibition of growth. Test concentration is well in excess of water solubility.
Executive summary:

No inhibition of growth. Test concentration is well in excess of water solubility.