Registration Dossier

Administrative data

Endpoint:
toxicity to terrestrial plants: long-term
Type of information:
migrated information: read-across based on grouping of substances (category approach)
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: GLP Guideline study
Cross-reference
Reason / purpose:
reference to same study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2000
Report Date:
2000

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 208 (Terrestrial Plants Test: Seedling Emergence and Seedling Growth Test)
GLP compliance:
yes (incl. certificate)

Test material

Reference
Name:
Unnamed
Type:
Constituent
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report): Genamin TA 100 D

Sampling and analysis

Analytical monitoring:
not specified

Test substrate

Vehicle:
no

Test organisms

open allclose all
Species:
Avena sativa
Plant group:
Monocotyledonae (monocots)
Species:
Raphanus sativus
Plant group:
Dicotyledonae (dicots)
Species:
Trifolium pratense
Plant group:
Dicotyledonae (dicots)

Study design

Test type:
early seedling growth toxicity test
Study type:
laboratory study
Substrate type:
artificial soil
Limit test:
no
Total exposure duration:
20 d

Test conditions

Test temperature:
21 - 24 °C
pH:
6.7 - 7.2
Details on test conditions:
TEST SYSTEM
- Test container (type, material, size): Flower pots, volume 300 mL
- Amount of soil: 200 mL
- No. of seeds per container: 5
- No. of replicates per treatment group: 4
- No. of replicates per control: 4



SOURCE AND PROPERTIES OF SUBSTRATE (if soil)
- Composition (if artificial substrate): LUFA Soil No. 2.3 8 batch no. Sp32299)
- Storage (condition, duration): at room temperature and protected from moisture until use


GROWTH CONDITIONS
- Photoperiod: 16 hours per day
- Light intensity and quality: 2241b ± 63 and 2523 ± 155 lux


EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : seedling and early stage of growth
Nominal and measured concentrations:
nominal concentrations of 1, 10 and 100 mg/kg dw
Reference substance (positive control):
no

Results and discussion

Effect concentrationsopen allclose all
Species:
Avena sativa
Duration:
20 d
Dose descriptor:
NOEC
Effect conc.:
>= 100 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
seedling emergence
Species:
Raphanus sativus
Duration:
20 d
Dose descriptor:
NOEC
Effect conc.:
>= 100 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
seedling emergence
Species:
Trifolium pratense
Duration:
20 d
Dose descriptor:
NOEC
Effect conc.:
>= 100 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
seedling emergence

Any other information on results incl. tables

The test substance did not cause effects on the tested higher plant species oat, red clover and radish when applied with 1, 10, 100 mg/kg dw. No significant differences were found , the NOECs for both emergence and biomass growth were >= 100 mg/kg

Applicant's summary and conclusion