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Ecotoxicological information

Long-term toxicity to aquatic invertebrates

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Administrative data

Endpoint:
long-term toxicity to aquatic invertebrates
Type of information:
migrated information: read-across based on grouping of substances (category approach)
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: GLP Guideline study with restictions
Cross-reference
Reason / purpose:
reference to same study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2002
Report Date:
2002

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 211 (Daphnia magna Reproduction Test)
GLP compliance:
yes (incl. certificate)

Test material

Reference
Name:
Unnamed
Type:
Constituent
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report): Tallowalkylamine

Test solutions

Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Dispersion treatment: Stock solution was 10 mg/L. The test item was applied to natural river water. This solution was treated for 30 minutes with ultrasound at 40 °C.

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Common name: Water flea
- Strain: Daphnia magna STRAUS (Clone 5)
- Source: Institut für Wasser-, Boden- und Lufthygiene (WaBoLu); Breeder DR.U.NOACK-LABORATORIEN, D-31157 Sarstedt, Germany
- Age at study initiation: 2 - 24 h old
- Feeding during test: The daphnids were not fed during the study.

HOUSING:
- Conditions: In 2-3 L glass vessels with approximately 1.8 L culture medium, at 21 °C (temperatures of 20 - 25 °C are tolerated), in an
incubator, 16 h illumination, illumination strength max. 20 μE⋅m-2 ⋅s-1.
- Culture medium: Elendt M4, according to ELENDT (1990), modified to a total hardness of 160 to 180 mg CaCO3/L.
- Type and amount of food: Feeding ad libitum with a mix of Desmodesmus subspicatus and Chlorella vulgaris, with an algae cell density of > 106 cells/mL.
- Feeding frequency: 5x weekly



Study design

Test type:
semi-static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
21 d

Test conditions

Test temperature:
20±2 °C
Nominal and measured concentrations:
Nominal concetrations: 0.013, 0.032, 0.08, 0.2, 0.5 mg/L
Details on test conditions:
TEST SYSTEM
- Test vessel:
- Material, size, fill volume: Glass beakers (5 cm ID x 8 cm H), 100 mL; Volume of the study medium is 50 mL
- No. of organisms per vessel: 1
- No. of vessels per concentration (replicates): 10
- No. of vessels per control (replicates): 10
- Renewal rate of test solution: 3 times per week ( monday, wednesday and friday)



TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: A natural river water of agricultural backround, middle reach of the river "Böhme", Lower-Saxony was used.
Test water parameter:
pH: 8.2
Conductivity [μS/cm]: 397
DOC [mg C/L]: 7.3
TOC [mg C/L]: 9.9
Ammonium-N [mg N/L]: 0.141
Nitrate-N [mg N/L]: 2.38
o-Phosphate-P [mg P/L]: 0.031
Total Phosphate [mg P/L]: 0.128
Suspended Matter [mg/L]: 17.4
Total Hardness [mg CO3/L]: 91.3
- Culture medium different from test medium: yes


OTHER TEST CONDITIONS
- Photoperiod: 16/8 hours light dark cycle
- Light intensity: Diffuse light, illumination range max. 20 μE⋅m-2 ⋅ s-1


EFFECT PARAMETERS MEASURED: reproduction and mortality


Reference substance (positive control):
yes
Remarks:
potassiumdichromate

Results and discussion

Effect concentrationsopen allclose all
Duration:
21 d
Dose descriptor:
NOEC
Effect conc.:
0.013 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
reproduction
Duration:
21 d
Dose descriptor:
LOEC
Effect conc.:
0.032 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
reproduction
Duration:
21 d
Dose descriptor:
EC50
Effect conc.:
0.24 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
reproduction
Results with reference substance (positive control):
- Results with reference substance valid? yes
- Relevant effect levels: EC50 = 2.1 (24h)

Any other information on results incl. tables

Table 1: Summary of all test item related effects in the test concentrations

Effects

Nominal concentrations (mg/L)

0.013

0.032

0.08

0.02

0.5

Reproduction rate

--

++

++

++

++

Stillborn juveniles

--

--

++

++

--

Aborded eggs

--

--

--

--

--

immobilization

--

--

--

--

++

Dry weight

--

--

--

--

++

Lenght

--

--

--

--

++

Males/winter eggs

--

--

--

--

--

Summary:

--

++

++

++

++

-- no significant effect

++ significant effect

Applicant's summary and conclusion