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Ecotoxicological information

Toxicity to soil macroorganisms except arthropods

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Administrative data

Endpoint:
toxicity to soil macroorganisms except arthropods: long-term
Type of information:
migrated information: read-across based on grouping of substances (category approach)
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: GLP Guideline study
Cross-reference
Reason / purpose:
reference to same study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2006
Report Date:
2006

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 222 (Earthworm Reproduction Test (Eisenia fetida/Eisenia andrei))
GLP compliance:
yes (incl. certificate)

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report): Amines, hydrogenated tallow

Test substrate

Vehicle:
yes
Details on preparation and application of test substrate:
- Method of mixing into soil: For application quartz sand was used as a vehicle. A mixture comprised of 10 g quartz sand with neccessary quantitiy of the test item was perpared per replicate. The mixture was throughly mixed with the pre-moistured soil with a long handled blender for at least four minutes. Afterwards the soil was moistured to 60 % of its maximum water holding capacity.

Test organisms

Test organisms (species):
Eisenia fetida
Animal group:
annelids
Details on test organisms:
TEST ORGANISM
- Common name: earthworm
- Source: own breeding, original source was the Bundesanstalt für Verbraucherschutz und Landwirtschaft (BVL)
- Age at test initiation (mean and range, SD): 2-12 month (the difference of age dit not deviate by more than one month)
- Weight at test initiation: 0.35 - 0.60 g

Housing:
- Housing conditions: Organisms were bred at the testing facility in covered plasitc vessels (volume 60 L) containing potting compost at 20 ± "°C in the dark

ACCLIMATION
- Acclimation period: 2 days

Study design

Study type:
laboratory study
Substrate type:
natural soil
Limit test:
no
Total exposure duration:
8 wk

Test conditions

Test temperature:
20 ± 2 °C
pH:
5.9 - 6.58
Moisture:
30 ± 3 %
Details on test conditions:
TEST SYSTEM
- Test container (material, size): plastic boxes with an inner diameter of 15 cm corresponding to a bottom surface area of 177 cm² and a height of 14 cm.
- Amount of soil or substrate: 600 g natural soil (dry weight)
- No. of organisms per container (treatment): 10
- No. of replicates per treatment group: 4
- No. of replicates per control: 8
- No. of replicates per vehicle control: 4

SOURCE AND PROPERTIES OF SUBSTRATE (if soil)
- Geographic location: LUFA 2.2 soil, Speyer
- Pesticide use history at the collection site: no pesticide treatment in the sampling year and 4 former years.
- Collection procedures: the soil was sampled 13 days prior to use and refrigerated at 4-7 °C in the testing facility until use.
- Sampling depth (cm): ca. 25
- Soil texture (if natural soil)
- % sand: ca 77
- % silt: ca 15
- % clay: 7.9
- Organic carbon (%): 2.33 ± 0.18
- Maximum water holding capacity (in % dry weigth): 49 ± 4.0


OTHER TEST CONDITIONS
- Photoperiod: 16 hours
- Light intensity: 400/800 lux


EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : mortality, weight and reproduction
Nominal and measured concentrations:
Nominal concatrations: control, 50, 100, 200, 500, 1000 mg/kg dry weight.
Reference substance (positive control):
yes
Remarks:
Carbendazim

Results and discussion

Effect concentrationsopen allclose all
Duration:
8 wk
Dose descriptor:
NOEC
Effect conc.:
200 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
reproduction
Remarks:
mortality and body weight
Duration:
8 wk
Dose descriptor:
LOEC
Effect conc.:
500 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
reproduction
Remarks:
mortality and body weight

Applicant's summary and conclusion

Executive summary:

Body length, eggs per worm and % of gravid worms are shown in Figure 1. Threshold

values (NOEC, LOEC) are given in Table 1. Mean values of the single replicates are

shown in Appendix B (Table I-IV).

Sodium tetrapropenyl benzene sulfonate: The range finding test showed that there

was no effect at 135 mg/Kg Sed (dw), whereas we found a total inhibition of all test

parameters at 500 mg/Kg Sed (dw). In order to render NOEC and LOEC more

precisely, smaller concentration steps were investigated in the main test. The results

of the main test agreed well with those of the range finding test. The NOEC was

found at 100, the LOEC at 200 mg/Kg Sed (dw).

Cocos dimethylamine: The results of the range finding test and the main test are

slightly different, however they are in the same order of magnitude (Fig. 1). We took

the results of the main test for calculations of NOEC and LOEC, because of the

higher number of replicates and thus higher statistical significance.

Di hardened tallow dimethyl ammonium chloride: Here we did not find an effect in the

range finding test up to the highest tested concentration of 1350 mg/Kg Sed (dw).

Thus we investigated higher concentrations (up to 5410 mg/Kg Sed (dw)) in the main

test. The NOEC-value was found at 1350, the LOEC-value at 2030 mg/Kg Sed (dw).

Tallow alkylamine: For this substance we did not find an effect up to the highest

tested concentration of 2030 mg/Kg Sed (dw). Here we reached the limit of solubility.

When the stock solution was added to the sediment, a white precipitate occurred at

high concentrations.