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Ecotoxicological information

Toxicity to microorganisms

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Administrative data

Endpoint:
activated sludge respiration inhibition testing
Type of information:
migrated information: read-across based on grouping of substances (category approach)
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Guideline study with acceptable restrictions
Cross-reference
Reason / purpose:
reference to same study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1989
Report Date:
1989

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
Deviations:
yes
Remarks:
no detailed documentation
GLP compliance:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report): Genamin TA 100 D

Sampling and analysis

Analytical monitoring:
no

Test solutions

Vehicle:
no

Test organisms

Test organisms (species):
activated sludge of a predominantly domestic sewage
Details on inoculum:
- Source of inoculum: Activated sludge from domestic STP Hildesheim, Germany
- Preparation of inoculum for exposure: After settling the sludge was washed twice with dechlorinated (autoclaved) tap water
- Initial biomass concentration: activated sludge dry weight: 1.9 g/L

Study design

Test type:
static
Water media type:
freshwater
Total exposure duration:
3 h

Test conditions

Test temperature:
20.6°C
Nominal and measured concentrations:
Nominal concentrations: 10, 50, 100, 200 mg/L
Details on test conditions:
TEST SYSTEM
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 2


TEST MEDIUM / WATER PARAMETERS
-Culture medium: synthetic sewage feed as described in OECD 209
- Dilution water: Dechlorinated tap water
- Intervals of water quality measurement: continuous measurement of oxygen content using a oxygen electrode


EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : inhibition of respiration rate
Reference substance (positive control):
yes
Remarks:
potassium dichromate (60 mg/L)

Results and discussion

Effect concentrationsopen allclose all
Duration:
3 h
Dose descriptor:
EC50
Effect conc.:
32 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate
Remarks on result:
other: 95% CL: 5.8 - 178 mg/L
Duration:
3 h
Dose descriptor:
EC10
Effect conc.:
7 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate
Duration:
3 h
Dose descriptor:
other: EC20
Effect conc.:
12 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate
Duration:
3 h
Dose descriptor:
other: EC80
Effect conc.:
90 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate
Details on results:
The controls had a deviation of 1.1%, thus the test is valid.
Results with reference substance (positive control):
- Results with reference substance valid? yes, 46.4% inhibition of respiration rate

Any other information on results incl. tables

Table 1: Respiration inhibition of Genamin TA 100 D

 

Test concentrations (mg/L)

Respiration rate

R (mgO2/L*h)

Inhibition (%)

Main test

10

50

100

200

23.7

7.8

4.4

2.0

11.7

70.9

83.6

92.6

Reference

60

14.4

46.4

Control

K1

K2

27.0

26.7

 

Applicant's summary and conclusion