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Ecotoxicological information

Toxicity to microorganisms

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Endpoint:
activated sludge respiration inhibition testing
Data waiving:
study scientifically not necessary / other information available
Justification for data waiving:
the study does not need to be conducted because the substance is found to be readily biodegradable and the applied test concentrations are in the range of concentrations that can be expected in the influent of a sewage treatment plant
Justification for type of information:
Study on the test substance is not necessary as valid conclusions regarding bacterial toxicity can be derived from RSS for the ready biodegradability test in this dossier.
Reason / purpose:
reference to same study
GLP compliance:
yes
Validity criteria fulfilled:
yes
Conclusions:
Results from a 14 d ready biodegradability study using a bacterial innoculum derived from sludge obtained from municipal waste water treatment plants in Japan, as well as from natural soil and waters, indicats that the substance is readily degradable. According to the data presented (see RSS) BOD at 14 days in the three triplicates containing 100 mg/L disodium tartrate exhibited a far greater demand than the control blank, containing sludge and dilution medium (all samples containing slude at 30 mg/L). Thereby it can be concluded that no effect upon respiratory activity of the bacteria was caused after 14d exposure by the amalogu disodium tartrate.
Executive summary:

Results from a 14 d ready biodegradability study using a bacterial innoculum derived from sludge obtained from municipal waste water treatment plants in Japan, as well as from natural soil and waters, indicats that the substance is readily degradable. According to the data presented (see RSS) BOD at 14 days in the three triplicates containing 100 mg/L disodium tartrate exhibited a far greater demand than the control blank, containing sludge and dilution medium (all samples containing slude at 30 mg/L). Thereby it can be concluded that no effect upon respiratory activity of the bacteria was caused after 14d exposure by the amalogu disodium tartrate. Thereby, considering the only effect detected is stimulation of respiration (the tartrate is degraded as a substrate for respiration of the sludge), a NOEC value of 100 mg/L fpr analogue substance disodium tartrate can be provided.

Endpoint:
toxicity to microorganisms
Type of information:
migrated information: read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
supporting study
Reliability:
4 (not assignable)
Rationale for reliability incl. deficiencies:
other: Only brief summary reported in IUCLID 4 report for Citric acid.
Qualifier:
no guideline followed
Guideline:
other: Not cited
GLP compliance:
not specified
Test organisms (species):
Pseudomonas putida
Test type:
not specified
Water media type:
not specified
Dose descriptor:
EC50
Effect conc.:
> 10 000 mg/L
Nominal / measured:
not specified
Conc. based on:
not specified
Basis for effect:
not specified
Validity criteria fulfilled:
not specified
Executive summary:

Toxicity study reported for effect of citric acid on Pseudomonas putida in IUCLID 4 dataset. Data insufficient to assess validity of EC50 reported as > 10 g/L, but overall is coherent with metabolic and other toxicological data which support to low toxicity and easy biodegradability of short chained dicarboxyllic acids by bacterial cultures.

Description of key information

According to the data presented (see RSS) BOD at 14 days in the three triplicates containing 100 mg/L disodium tartrate exhibited a far greater demand than the control blank, containing sludge and dilution medium (all samples containing slude at 30 mg/L). Thereby it can be concluded that no effect upon respiratory activity of the bacteria was caused after 14d exposure by the analogue disodium tartrate.

Key value for chemical safety assessment

EC10 or NOEC for microorganisms:
100 mg/L

Additional information

Results from a 14 d ready biodegradability study using a bacterial innoculum derived from sludge obtained from municipal waste water

treatment plants in Japan, as well as from natural soil and waters, indicats that the substance is readily degradable. According to the data

presented (see RSS) BOD at 14 days in the three triplicates containing 100 mg/L disodium tartrate exhibited a far greater demand than

the control blank, containing sludge and dilution medium (all samples containing slude at 30 mg/L). Thereby it can be concluded that no

effect upon respiratory activity of the bacteria was caused after 14d exposure by the analogue disodium tartrate. Thereby, considering the

only effect detected is stimulation of respiration (the tartrate is degraded as a substrate for respiration of the sludge), a NOEC value of

100 mg/L fpr analogue substance disodium tartrate can be provided.

In addition, a toxicity study was identified reporting effects of citric acid on Pseudomonas putida in IUCLID 4 dataset. Data insufficient to

assess validity of EC50 reported as > 10 g/L, but overall is coherent with metabolic and other toxicological data which support to low

toxicity and easy biodegradability of short chained dicarboxyllic acids by bacterial cultures.