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EC number: 204-649-2
CAS number: 123-76-2
The ability of the test item to induce
skin sensitisation in the CBA/JN mouse, using the Local Lymph Node Assay
(LLNA: BrdU-ELISA method) according to the OECD Guideline 442b.
In the main assay, the test item was
topically administered at the concentrations of 25, 10 and 5 % (w/w), in
acetone:olive oil 4:1 (v/v) for three consecutive days. After one day of
no treatment, BrdU solution was injected inter-peritoneally. Twenty four
hours after BrdU injection, the animals were killed and the auricular
lymph nodes were rapidly excised, pooled on individual basis and
individually collected. Cell suspensions were prepared for the
evaluation of proliferation at lymph node.
An increase in cell proliferation of
draining lymph nodes was observed in the high, medium and low dose
groups with SI of 2.05, 1.88 and 1.31 respectively, indicating that the
test item may elicit a sensitisation response. Dunnett’s test showed a
statistically significant difference between mid- and high dose groups,
when compared to the negative control group.
The substance is considered to be a skin
sensitiser as the SI is higher than 1.6 in the mid and high dose group.
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