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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

additional ecotoxicological information
Type of information:
experimental study
Adequacy of study:
key study
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
comparable to guideline study with acceptable restrictions

Data source

Reference Type:
Ecotoxicity studies of the levulinate ester series
Lomba L, Muñiz, S., Pino M., Navarro, E. and Giner, B.
Bibliographic source:
Ecotoxicology (2014) 23:1484–1493

Materials and methods

Test guideline
according to guideline
other: UNE-EN-ISO 11348-3 (2007) Water quality: determination of the inhibitory effect of water samples on the light emission of Vibrio fischeri (Luminescent bacteria test)
Type of study / information:
Determination of the inhibitory effect of water samples on the light emission of Vibrio fischeri (Luminescent bacteria test).

Test material

Constituent 1
Chemical structure
Reference substance name:
4-oxovaleric acid
EC Number:
EC Name:
4-oxovaleric acid
Cas Number:
Molecular formula:
4-oxopentanoic acid

Results and discussion

Any other information on results incl. tables

EC50 = 5687±1325 mg/l.

Applicant's summary and conclusion

EC50 (30 min) = 5687±1325 mg/l.
Executive summary:

The inhibition of bioluminescence of Vibrio fischeri bacteria was assessed according to the UNE-EN-ISO 11348-3. The lyophilized V. fischeri were exposed to several dilutions for each of the chemical were prepared using a 2 % NaCl stock solution. The different concentrations ranges assessed for this bioassay were 0–20000 mg/l.

The solution equilibrated for 10 min at 15 °C, and subsequently, the first measurements were taken to obtain the initial luminescence; then 0.5 ml of each dilution to be tested was added to the cuvette. The inhibition of luminescence was measured after 30 min.

EC50 (30 min) = 5687±1325 mg/l.