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Diss Factsheets

Environmental fate & pathways

Biodegradation in water: screening tests

Administrative data

Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Study period:
23.december 2003 - 30. april 2004
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: GLP guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2004
Report date:
2004

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
Deviations:
no
GLP compliance:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
[(2S,3R,5S)-3-[(4-methylbenzoyl)oxy]-5-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)tetrahydrofuran-2-yl]methyl 4-methylbenzoate
EC Number:
608-497-1
Cas Number:
3056-13-1
Molecular formula:
C26 H26 N2 O7
IUPAC Name:
[(2S,3R,5S)-3-[(4-methylbenzoyl)oxy]-5-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)tetrahydrofuran-2-yl]methyl 4-methylbenzoate

Study design

Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge, domestic, non-adapted
Details on inoculum:
Activated sludge was collected from a sewage treatment works A-2500 Baden, which wastewater catchment is predominantly domestic.
On arrival in the laboratory, the sample was aerated by means of a filtered compressed air before being used for the study.
The suspended solids concentration was determined by filtering a 5 mL sample through a pre-dried and pre-weighed glass filter (Whatman GF/C). The filter with solids were dried at 105 °C and re-weighed and the sludge solids determined by difference.
The inoculum was not acclimatised or adapted to the test substance before exposure to the test substance in this study.
Duration of test (contact time):
28 d
Initial test substance concentration
Initial conc.:
22.98 mg/L
Based on:
test mat.
Parameter followed for biodegradation estimation
Parameter followed for biodegradation estimation:
CO2 evolution
Reference substance
Reference substance:
benzoic acid, sodium salt

Results and discussion

% Degradation
Parameter:
% degradation (CO2 evolution)
Value:
30.6
Sampling time:
28 d
Details on results:
Degradation kinetic:
Day 1 = 1%
Day 8 = 10.9%
Day 20 = 21.1%
Day 28 = 30.6%

BOD5 / COD results

Results with reference substance:
Degredation kinetic:
Day 2 = 25.5%
Day 8 = 67.8%
Day 28 = 96.4%

Applicant's summary and conclusion

Interpretation of results:
other: not readily biodegradable
Executive summary:

Aim and methods

The biodegradability of the test substance was analysed by the determination of carbon dioxide

evolution at frequent intervals over a 28 d period. The method used was that described in

Part C.4-C of the EC Commission Directive 92/69/EEC and in the OECD Guideline 301B.

 

Results

The reference substance sodium benzoate was degraded by 74.8% within 10 days.

Degradation in the toxicity control, which contained sodium benzoate and the test substance,

was not indicative of an inhibition of the microbial activity by the test substance.

 

The carbon dioxide evolution from the test substance reached a maximum of 30.6% after 28

 days of incubation.

 

Classification

According to the results of this study, the test substance can not be classified as readily

biodegradable.