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EC number: 451-620-7
CAS number: 352230-22-9
Biodegradation of the
The results are presented in
Tables 2 and 3.
The % biodegradation of the
reference item sodium benzoate was calculated based on the theoretical
oxygen demand of 1.67 mg O2/mg (ThOD).
In the procedure controls, the
reference item was degraded by an average of 87% by exposure Day 14,
confirming suitability of the activated sludge.
Biodegradation in the
In the toxicity control,
containing both test item and the reference item, the course of
biodegradation over 28 day exposure period was identical to that in the
two procedure controls containing the reference item only (See Table 2).
Thus, the test item has obviously
no inhibitory effect on activated sludge microorganisms.
Measurement of pH:
The results are presented in Table
The pH measured in all flasks at
the start of the test was 7.4. At the end of exposure (Day 28), pH
values were between 7.3 and 7.8.
The tabulated values represent
rounded results obtained by calculation using exact raw data
Table 2: Oxygen consumption in the
Cumulative oxygen consumption (mg/L)
Test item (flask No.)
Inoculum control (flask No.)
Procedure control (flask No.)
Abiotic control (flask No.)
Toxicity control (flask No.)
Table 3: Biodegradation of the
reference item in the procedure and toxicity controls
Procedure control (Flask No.)
Toxicity control (Flask No.)
Mean (Day 28)
*Corrected for the mean oxygen
uptake of the inoculum controls
Table 4: pH values at the start
and at the end of the test
Biodegradation in water: screening
tests: 2 - 4% in 28 days (O2 consumption, OECD 301F)
% Biodegradation of 2 - 4% in 28
days (O2 consumption) was obtained for the submission
substance in accordance with OECD Test Guideline 301F and in compliance
with GLP. The result is considered to be reliable.
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