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Ecotoxicological information

Short-term toxicity to fish

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Description of key information

In the key study, a 96-hour toxicity study with Medaka larvae (Oryzias latipes), the 96h-LC50 was found to be 0.95 mg/l.

Key value for chemical safety assessment

LC50 for freshwater fish:
0.95 mg/L

Additional information

The toxicity of HHCB towards Medaka larvae (Oryzias latipes) was investigated during a 96 -hour test. The larvae were exposed to concentrations of HHCB ranging from 0.60 -1.5 mg/l HHCB. The test solutions were refreshed on a daily basis. DMSO was used as a solvent carrier and a control with 0.1% DMSO was therefore included. The 96h-LC50 was found to be 0.95 mg/l (95% CL: 0.91 -1.01 mg/l).

In a supporting study, the 96h-LC50 was derived from a 21-d prolonged toxicity test with bluegill sunfish (Lepomis macrochirus). This test was carried out under GLP, according to OECD Test Guideline 204 under flow-through conditions (Wüthrich 1996). The validity criteria were met considering mortality in the control being < 10%; the dissolved oxygen was above 60% of air saturation and; there was a steady dose-effect relation. The data were also used to derive the 4d-LC50: 1.36 mg/l, with 95%-confidence limits 1.14 - 1.63 mg/l. Usually fish are not fed in a short term test. Feeding is not expected to reduce the toxicity of HHCB to fish. The information can be used for acute toxicity for deriving an LC50 result.