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Ecotoxicological information

Toxicity to soil macroorganisms except arthropods

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Description of key information

Key value for chemical safety assessment

Long-term EC10, LC10 or NOEC for soil macroorganisms:
910 mg/kg soil dw

Additional information

Eisenia fetida were exposed to Amides, C18-unsatd., N-[3-(dimethylamine)propyl] according to OECD Guideline 222 and GLP requirements. A range of concentrations composing of 0, 62.5, 125, 250, 500 and 1000 mg/kg soil dry weight corresponding to 56.9, 113.8, 227.5, 455 and 910 mg a.i./kg soil dry weight was used. The adult worms were between 8 and 9 months old and were exposed to the test substance for 56 days. The substrate used was artificial soil. There were 6 treatment groups with 4 replicates for the test item treatments and 8 replicates for the control with 10 worms each. The worms were assessed for adult worm mortality, behavioural effects and biomass development after 28 days of exposure. The reproduction rate was assessed after an additional 28 days.

All study validity criteria were met. No mortality was observed in any treatment group. The body weight change of the earthworms after 4 weeks exposure was not statistically significant different compared to the control up to and including the highest test concentration of 1000 mg test item/kg soil dry weight (Dunnett's test,α = 0.05). The reproduction rates after 8 weeks exposure to Amides, C18-unsatd., N-[3-(dimethylamine)propyl] were not significantly different compared to the control up to and including the highest test concentration of 1000 mg test item/kg soil ( Dunnett's t-test,α = 0.05). No behavioural abnormalities were observed in any of the treatment groups.