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Ecotoxicological information

Endpoint summary

Administrative data

Description of key information

Short term toxicity to fish:

This study was conducted to assess the effect of the test compound on fish Danio rerio. Test conducted in accordance with OECD Guideline 203 (Fish, Acute Toxicity Test). The test solution was prepared by dissolving 1 g of the test substance in 10 liters deionized water with continuous stirring. Test conducted under the static system by using the limit concentration of 100 mg/l for 96 hrs. The test vessels used were all glass bowl aquaria. The fishes were found to be freely swimming in the bowl aquaria without showing any abnormal symptoms. No mortalities were observed at the test concentration of 100 mg/l during 96 hours of exposure. No mortalities were found in the control aquaria. The median lethal concentration (LC50) for test chemical on Danio rerio in a 96 hours study on the basis of mortality effect was observed to be >100 mg/L as NOEC determine at 100 mg/l. Thus chemical consider to be nontoxic and not classified as per the CLP classification criteria.

 

Long term toxicity to fish:

Based on the prediction done using ECOSAR version 1.1, the long term toxicity on fish was predicted for test substance. On the basis of effects observed in a freshwater system, the NOEC value for the substance is estimated to be 69.556 mg/l for fish for 28 days of exposure duration., it can be concluded that the test chemical can be considered as non-toxic to fish at environmentally relevant concentrations and can be considered not-classified as per the CLP classification criteria. 

 

Short term toxicity to aquatic invertebrates:

Aim of this study was to assess the short term toxicity of test chemical to aquatic invertebrate daphnia magna. Study was performed according to the OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test) in a static system for the total exposure period of 48 hrs. The stock solution 400 mg/l was prepared by dissolving white crystalline substance in reconstituted water. Test solutions of required concentration as were prepared by mixing the stock solution of the test sample in reconstituted test water. 0, 200 and 400 mg/l concentrations were used in the study. Effects on immobilisation were observed for 48 hours. With the test substance one positive control Potassium dichromate (K2Cr2O7) was also run simultaneously. After the exposure of chemical, effect concentration EC50 was calculated using nonlinear regression by the software Prism 4.0. The median effective concentration (EC50) for the test substance, in Daphnia magna was determined to be > 400 mg/L on the basis of mobility inhibition effects in a 48 hour study. Based on the EC50 value, substance is likely to be non-hazardous to aquatic invertebrate and cannot classified as per the CLP classification criteria.

 

Long term toxicity to aquatic invertebrates:

Based on the prediction done using ECOSAR version, the long term toxicity on aquatic invertebrate was predicted for test substance. On the basis of effects observed in a freshwater system, the NOEC value for the substance is estimated to be 31.138 mg/l for aquatic invertebrate for 21 days of exposure duration. Thus, it can be concluded that the test chemical can be considered as non-toxic to aquatic invertebrate at environmentally relevant concentrations and can be considered not-classified as per the CLP classification criteria.

 

Toxicity to algae and cyanobacteria:

Aim of this study was to evaluate the nature of test chemical when comes in contact with the test organism Desmodesmus subspicatus (previous name: Scenedesmus subspicatus). Test was conducted according to the OECD guideline 201. Limit test performed using 100 mg/l. The stock solution 100 mg/l was prepared by dissolving white crystalline substance in OECD growth medium. With the test substance one positive control Potassium dichromate (K2Cr2O7) was also run simultaneously. After the exposure of chemical, effect concentration EC50 was calculated using nonlinear regression by the software Prism 4.0. Effect on the growth of algae was determine after an exposure period of 72 hrs. Based on the growth rate inhibition of algae Desmodesmus subspicatus (previous name: Scenedesmus subspicatus) due to the exposure of chemical for 72 hrs, only 2 % inhibition were observed at 100 mg/l. Thus based on the above effect, chemical consider to be non-hazardous to aquatic algae and cannot be classified as per the CLP classification criteria.

 

Toxicity to microorganism:

1st study: Based on the growth rate inhibition of Pseudomonas putida by the test chemical exposure for 18 hrs, the EC10 was determine at 2050 mg/l.

2nd study: Based on the effect observed on Mycobacterium smegmatis by the test chemical, the EC0 was determine at 9707 mg/l.

Thus based on the above effects, chemical toxicity ranges from 2050 mg/l to 9707 mg/l.

Additional information

Short term toxicity to fish:

This study was conducted to assess the effect of the test compound on fish Danio rerio. Test conducted in accordance with OECD Guideline 203 (Fish, Acute Toxicity Test). The test solution was prepared by dissolving 1 g of the test substance in 10 liters deionized water with continuous stirring. Test conducted under the static system by using the limit concentration of 100 mg/l for 96 hrs. The test vessels used were all glass bowl aquaria. The fishes were found to be freely swimming in the bowl aquaria without showing any abnormal symptoms. No mortalities were observed at the test concentration of 100 mg/l during 96 hours of exposure. No mortalities were found in the control aquaria. The median lethal concentration (LC50) for test chemical on Danio rerio in a 96 hours study on the basis of mortality effect was observed to be >100 mg/L as NOEC determine at 100 mg/l. Thus chemical consider to be nontoxic and not classified as per the CLP classification criteria.

 

Long term toxicity to fish:

Based on the prediction done using ECOSAR version 1.1, the long term toxicity on fish was predicted for test substance. On the basis of effects observed in a freshwater system, the NOEC value for the substance is estimated to be 69.556 mg/l for fish for 28 days of exposure duration., it can be concluded that the test chemical can be considered as non-toxic to fish at environmentally relevant concentrations and can be considered not-classified as per the CLP classification criteria. 

 

Short term toxicity to aquatic invertebrates:

Aim of this study was to assess the short term toxicity of test chemical to aquatic invertebrate daphnia magna. Study was performed according to the OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test) in a static system for the total exposure period of 48 hrs. The stock solution 400 mg/l was prepared by dissolving white crystalline substance in reconstituted water. Test solutions of required concentration as were prepared by mixing the stock solution of the test sample in reconstituted test water. 0, 200 and 400 mg/l concentrations were used in the study. Effects on immobilisation were observed for 48 hours. With the test substance one positive control Potassium dichromate (K2Cr2O7) was also run simultaneously. After the exposure of chemical, effect concentration EC50 was calculated using nonlinear regression by the software Prism 4.0. The median effective concentration (EC50) for the test substance, in Daphnia magna was determined to be > 400 mg/L on the basis of mobility inhibition effects in a 48 hour study. Based on the EC50 value, substance is likely to be non-hazardous to aquatic invertebrate and cannot classified as per the CLP classification criteria.

 

Long term toxicity to aquatic invertebrates:

Based on the prediction done using ECOSAR version, the long term toxicity on aquatic invertebrate was predicted for test substance. On the basis of effects observed in a freshwater system, the NOEC value for the substance is estimated to be 31.138 mg/l for aquatic invertebrate for 21 days of exposure duration. Thus, it can be concluded that the test chemical can be considered as non-toxic to aquatic invertebrate at environmentally relevant concentrations and can be considered not-classified as per the CLP classification criteria.

 

Toxicity to algae and cyanobacteria:

Based on the experimental studies for the test chemical, studies were reviewed and mention as below:

In the first experimental study toxicity were evaluated. Aim of this study was to evaluate the nature of test chemical when comes in contact with the test organism Desmodesmus subspicatus (previous name: Scenedesmus subspicatus). Test was conducted according to the OECD guideline 201. Limit test performed using 100 mg/l. The stock solution 100 mg/l was prepared by dissolving white crystalline substance in OECD growth medium. With the test substance one positive control Potassium dichromate (K2Cr2O7) was also run simultaneously. After the exposure of chemical, effect concentration EC50 was calculated using nonlinear regression by the software Prism 4.0. Effect on the growth of algae was determine after an exposure period of 72 hrs. Based on the growth rate inhibition of algae Desmodesmus subspicatus (previous name: Scenedesmus subspicatus) due to the exposure of chemical for 72 hrs, only 2 % inhibition were observed at 100 mg/l. Thus based on the above effect, chemical consider to be non-hazardous to aquatic algae and cannot be classified as per the CLP classification criteria.

 

First study was supported by the second study. The effect of test substance was studied on the growth of fresh water green alga Chlorella vulgaris. The study was conducted following OECD guideline 201- Alga growth inhibition test. The test concentration chosen for the study were 6.25 mg/l, 12.5 mg/l, 25 mg/l, 50 mg/l, 100 mg/l and 200 mg/l were prepared using stock solution of the test substance using de-ionized water. The green alga was exposed to the test concentration for a period of 72 hours to observe average specific growth rate and % growth inhibition under the effect of test substance. After 72 hours of exposure to test chemical to various nominal test concentration, EC25 was observed to be at 200 mg/L on the basis of growth rate inhibition, graphically through probit analysis. Thus based on the effect concentration, chemical consider to be nontoxic and not classified as per the CLP classification criteria.

Thus based on the above both studies, chemical consider to be nontoxic and not classified as per the CLP classification criteria.

Toxicity to microorganism:

Based on the experimental studies for the test chemical and structurally and functionally similar read across chemicals, studies have been were reviewed and mention as below:

 

Objective of this study was to determine the effect of test chemical on the growth of microorganism. Test conducted for 18 hrs. Based on the growth rate inhibition of Pseudomonas putida by the test chemical exposure for 18 hrs, the EC10 was determine at 2050 mg/l.

Above first study was supported by the second study from handbook. Aim of this study was to determine the effect of test chemical on the growth of microorganism Mycobacterium smegmatis. Based on the effect observed on Mycobacterium smegmatis by the test chemical, the EC0 was determine at 9707 mg/l.

Thus based on the above effects, chemical toxicity ranges from 2050 mg/l to 9707 mg/l.

Hence on the basis of effects observation on fish, invertebrates and algae, chemical consider to be nontoxic and not classified as per the CLP classification criteria.