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Short-term toxicity to fish

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Reference
Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study
Justification for type of information:
Experimental result using OECD recommended guidelines
Qualifier:
according to
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
Principles of method if other than guideline:
This study was conducted to assess the effect of the test compound on fish Danio rerio.
GLP compliance:
no
Specific details on test material used for the study:
Degree of purity/content : 99.41%
Physical state/appearance : Solid

STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
Storage conditions : Room temperature 30°C
Stability : Substance is stable under experimental conditions
Solubility in water : Soluble
Stability in water : Stable
Analytical monitoring:
no
Vehicle:
no
Details on test solutions:
Preparation of the test solutions/dispersions : The test solution was prepared by dissolving 1 g of the test substance in 10 liters deionized water with continuous stirring.
Test water : Deionized water was used.
Conductivity (µS/cm): 0.5 to 1
pH: 7
Test organisms (species):
Danio rerio (previous name: Brachydanio rerio)
Details on test organisms:
TEST ORGANISM
- Common name:Zebra Fish
- Source: Ashish Fish Center, Dharampeth, Nagpur, MS (India)
- Age at study initiation (mean and range, SD): 3-4 months
- Length at study initiation (length definition, mean, range and SD): average 2.3 cm
- Weight at study initiation (mean and range, SD): 0.203 g (approx.)
- Housing :The fishes were kept in a static tank in tap water, under natural conditions along with proper feed and aeration.
- Duration of housing : 15 days
- Oxygen content : 7.4
- pH : Between 6-7
- Water temperature : 29 °C
- Mortality (%) :0%
- Diet : Standard brand feed, once daily
Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
96 h
Test temperature:
29°C
pH:
6-7
Dissolved oxygen:
7.4
Nominal and measured concentrations:
100 mg/L
Details on test conditions:
TEST SYSTEM
- Test vessel: The test vessels used were all glass bowl aquaria of 12” each.
- Aeration: Aeration was provided 1 hr before the starting of experiment and also during the experiment when required.
- No. of organisms per vessel: 8 fishes

OTHER TEST CONDITIONS
- Photoperiod:12 hours light and 12 hours darkness

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : LC50 was determined.Observations (mortality, visible symptoms, pH, Temperature, dissolved oxygen content) were recorded after 1st, 2nd, 3rd and 4th day of the start of the experiment.

TEST CONCENTRATIONS : Test was conducted at 100 mg/L to observe the adverse effect/ no effect of test substance on fresh water fish.
Reference substance (positive control):
no
Key result
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: LC0 was observed at 100 mg/l
Details on results:
The fishes were found to be freely swimming in the bowl aquaria without showing any abnormal symptoms. No mortalities were observed at the test concentration of 100 mg/l during 96 hours of exposure. No mortalities were found in the control aquaria.

OBSERVATIONS:

Table 1. Mortality and symptoms

 

Nominal conc. (mg/l)

Number of fish

Mortality (cumulated) after

 

1stDay

2ndDay

3rdDay

4thDay

0 (control)

8

0

0

0

0

100

8

0

0

0

0

 

Nominal conc. (mg/l)

Number of fish

Symptoms after

1stDay

2ndDay

3rdDay

4thDay

0 (control)

8

NS8

NS8

NS8

NS8

100

8

NS8

NS8

NS8

NS8

 

Explanation of symptoms:

A: apathy

N: narcotic-like state

C: swimming near water surface

D: swimming at the bottom of bowl

M: Slow movements compared to control

NS: No abnormal symptoms

Number behind symbol for symptom= number of affected fish

 

Table 2. pH and oxygen content:

 

Nominal conc. (mg/l)

Number of fish

pH Readings after

1stDay

2ndDay

3rdDay

4thDay

0 (control)

8

6.4

6.8

6.7

6.1

100

8

6.5

6.6

6.5

7.3

 

 

Nominal conc. (mg/l)

Number of fish

Oxygen content (mg/l) after

1stDay

2ndDay

3rdDay

4thDay

0 (control)

8

7.4

7.1

6.1

6.5

100

8

7.5

7.2

6.2

6.5

 

Table 3. Test temperature:

Nominal conc. (mg/l)

Number of fish

Temperature (°C) after

1stDay

2ndDay

3rdDay

4thDay

0 (control)

8

28

28

29

30

100

8

28

28

29

30

Validity criteria fulfilled:
yes
Conclusions:
The median lethal concentration (LC50) for test chemical on Danio rerio in a 96 hours study on the basis of mortality effect was observed to be >100 mg/L as NOEC determine at 100 mg/l.

Executive summary:

This study was conducted to assess the effect of the test compound on fish Danio rerio. Test conducted in accordance with OECD Guideline 203 (Fish, Acute Toxicity Test). The test solution was prepared by dissolving 1 g of the test substance in 10 liters deionized water with continuous stirring. Test conducted under the static system by using the limit concentration of 100 mg/l for 96 hrs. The test vessels used were all glass bowl aquaria. The fishes were found to be freely swimming in the bowl aquaria without showing any abnormal symptoms. No mortalities were observed at the test concentration of 100 mg/l during 96 hours of exposure. No mortalities were found in the control aquaria. The median lethal concentration (LC50) for test chemical on Danio rerio in a 96 hours study on the basis of mortality effect was observed to be >100 mg/L as NOEC determine at 100 mg/l. Thus chemical consider to be nontoxic and not classified as per the CLP classification criteria.

Description of key information

This study was conducted to assess the effect of the test compound on fish Danio rerio. Test conducted in accordance with OECD Guideline 203 (Fish, Acute Toxicity Test). The test solution was prepared by dissolving 1 g of the test substance in 10 liters deionized water with continuous stirring. Test conducted under the static system by using the limit concentration of 100 mg/l for 96 hrs. The test vessels used were all glass bowl aquaria. The fishes were found to be freely swimming in the bowl aquaria without showing any abnormal symptoms. No mortalities were observed at the test concentration of 100 mg/l during 96 hours of exposure. No mortalities were found in the control aquaria. The median lethal concentration (LC50) for test chemical on Danio rerio in a 96 hours study on the basis of mortality effect was observed to be >100 mg/L as NOEC determine at 100 mg/l. Thus chemical consider to be nontoxic and not classified as per the CLP classification criteria.

Key value for chemical safety assessment

LC50 for freshwater fish:
100 mg/L

Additional information

This study was conducted to assess the effect of the test compound on fish Danio rerio. Test conducted in accordance with OECD Guideline 203 (Fish, Acute Toxicity Test). The test solution was prepared by dissolving 1 g of the test substance in 10 liters deionized water with continuous stirring. Test conducted under the static system by using the limit concentration of 100 mg/l for 96 hrs. The test vessels used were all glass bowl aquaria. The fishes were found to be freely swimming in the bowl aquaria without showing any abnormal symptoms. No mortalities were observed at the test concentration of 100 mg/l during 96 hours of exposure. No mortalities were found in the control aquaria. The median lethal concentration (LC50) for test chemical on Danio rerio in a 96 hours study on the basis of mortality effect was observed to be >100 mg/L as NOEC determine at 100 mg/l. Thus chemical consider to be nontoxic and not classified as per the CLP classification criteria.