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Toxicological information

Acute Toxicity: oral

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Administrative data

Endpoint:
acute toxicity: oral
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
data from handbook or collection of data
Justification for type of information:
Data is from publication

Data source

Referenceopen allclose all

Reference Type:
publication
Title:
To determine acute oral toxicity of test chemical in mice
Author:
Kazuo Hashimoto et. al.
Year:
1981
Bibliographic source:
Archives of Toxicology
Reference Type:
review article or handbook
Title:
ACUTE ORAL TOXICITY STUDY IN MICE
Author:
Richard J. Lewis
Year:
2012
Bibliographic source:
Sax's Dangerous Properties of Industrial Materials, 12th Edition, 5 Volume Set,2012
Reference Type:
other: Authoritive database
Title:
Acute oral toxicity study of test chemical in mice
Author:
U.S. National Library of Medicine
Year:
2018
Bibliographic source:
Chemidplus Database,U.S. National Library of Medicine,2018
Reference Type:
secondary source
Title:
acute oral toxicity study of test chemical in mice
Author:
RTECS
Year:
2018
Bibliographic source:
RTECS (registry of toxic effect of chemical substance database), 2018

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
other: as mentioned below
Principles of method if other than guideline:
The acute toxicity of the test chemical by oral administration in mice was determined according to Weil (1952), using four animals per dosage level and four different doses.
GLP compliance:
not specified
Test type:
other: not specified
Limit test:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Name of test material (IUPAC name): 2-Propenamide, N-(1,1-dimethylethyl)-
- Common name: N-tert-butylacrylamide
- Molecular formula: C7H13NO
- Molecular weight: 127.1857g/mol
- Smiles notation: CC(C)(C)NC(=O)C=C
- InChl: 1S/C7H13NO/c1-5-6(9)8-7(2,3)4/h5H,1H2,2-4H3,(H,8,9)
- Substance type: Organic

Test animals

Species:
mouse
Strain:
other: ddY
Sex:
male
Details on test animals and environmental conditions:
TEST ANIMALS
- Age at study initiation: 5-6 weeks
- Weight at study initiation: 29 ± 2.2 g body weight
- Housing: Plastic cages containing wooden flakes.
- Diet (e.g. ad libitum): laboratory chow ad libitum
- Water (e.g. ad libitum): water ad libitum

Administration / exposure

Route of administration:
oral: unspecified
Vehicle:
DMSO
Details on oral exposure:
no data available
Doses:
712.23 - 1233.7 mg/kg bw
No. of animals per sex per dose:
total:4 male mice
Control animals:
not specified
Details on study design:
no data available
Statistics:
The acute toxicity of the test compounds by oral administration in mice was determined according to Weil (1952).

Results and discussion

Preliminary study:
no data available
Effect levels
Sex:
male
Dose descriptor:
LD50
Effect level:
941.174 mg/kg bw
Based on:
test mat.
95% CL:
712.23 - 1 233.7
Remarks on result:
other: 50% mortality was observed
Mortality:
50% Mortality was observed in treated mice at 941.174 mg/kg bw
Clinical signs:
no data available
Body weight:
no data available
Gross pathology:
no data available
Other findings:
no data available

Applicant's summary and conclusion

Interpretation of results:
Category 4 based on GHS criteria
Conclusions:
The lethal concentration (LD50) value for acute oral toxicity test was considered to be 914.174 mg/kg bw (95% confidence limit:712.23 - 1233.7 mg/kg),when 4 male ddy strain mice were treated with test chemical orally.
Executive summary:

Acute oral toxicity study was performed in 4 male ddy strain mice using test chemical at dose concentration of 712.23 - 1233.7 mg/kg bw.DMSO was used as vehicle.50% mortality was observed at dose 914.174 mg/kg bw (95% confidence limit:712.23 - 1233.7 mg/kg).Hence,LD50 value was considered to be 914.174 mg/kg bw,when mice were treated with test chemical orally.