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EC number: 211-889-1 | CAS number: 705-86-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
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- Flash point
- Auto flammability
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- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to microorganisms, other
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Data is from peer reviewed journal
- Justification for type of information:
- Data is from peer reviewed journal
- Qualifier:
- according to guideline
- Guideline:
- other: as mentioned below
- Principles of method if other than guideline:
- Toxicity to microorganism study was carried out.
- GLP compliance:
- not specified
- Analytical monitoring:
- not specified
- Vehicle:
- yes
- Remarks:
- dimethyl sulfoxide (DMSO) was used as a vehicle.
- Test organisms (species):
- Tetrahymena pyriformis
- Test type:
- static
- Water media type:
- freshwater
- Total exposure duration:
- 40 h
- Details on test conditions:
- TEST SYSTEM
- Test vessel: Flasks were used as a test vessel.
- No. of vessels per concentration (replicates): Duplicates
- Other: Two control vessels, one control with no test chemical but inoculated with T. pyriformis, and the other, a blank control, which has neither test chemical nor test organism were run simultaneously during the study.
EFFECT PARAMETERS MEASURED (with observation intervals if applicable): Population density of test organism was measured spectrophotometrically at 540 nm.
TEST CONCENTRATIONS
- Range finding study: yes, range finding study was performed in duplicates prior to the initiation of study. - Reference substance (positive control):
- not specified
- Key result
- Duration:
- 40 h
- Dose descriptor:
- other: IGC50
- Effect conc.:
- 204.64 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth inhibition
- Reported statistics and error estimates:
- The 50% growth inhibitory concentrations, IGC50, were determined by Probit Analysis of Statistical Analysis System (SAS) software
- Validity criteria fulfilled:
- not specified
- Conclusions:
- Based on effect on growth inhibition of the test organism Tetrahymena pyriformis, the 40 hr IGC50 value was determined to be 204.64 mg/l.
- Executive summary:
Toxicity to microorganism study was performed using Tetrahymena pyriformis as a test organism. The study was carried out under static conditions. Stock solutions of test chemical were prepared in dimethyl sulfoxide. Exact test chemical concentrations used for the study was not known, but each test replicate consists of six to eight different concentrations. Range finding study was performed in duplicates prior to the initiation of study. Flasks were used as a test vessel. To each test flask were added test organism and a solution of test chemical. Two control vessels, one control with no test chemical but inoculated with T. pyriformis, and the other, a blank control, which has neither test chemical nor test organism were run simultaneously during the study. All test concentrations experiments were performed in duplicates. Test conditions allow for 8-9 cell cycles in control cultures. Only replicates with control-absorbency values > 0.6 but < 0.75 were used for the analysis. Population density of test organism was measured spectrophotometrically at 540 nm. The 50% growth inhibitory concentrations, IGC50, were determined by Probit Analysis of Statistical Analysis System (SAS) software. On the basis of the effect on growth inhibition of the test organism Tetrahymena pyriformis, the 40 hr IGC50 value was determined to be 204.64 mg/l.
Reference
Table: Toxicity of test chemical result.
Chemical |
Log 1/IGC50 |
logKow (1-octanol/water) |
Test chemical |
-0.08 |
2.47 |
Description of key information
Toxicity to microorganism study was performed using Tetrahymena pyriformis as a test organism (T. W. Schultz et. al., 1999 and authoritative database, 2012). The study was carried out under static conditions. Stock solutions of test chemical were prepared in dimethyl sulfoxide. Exact test chemical concentrations used for the study was not known, but each test replicate consists of six to eight different concentrations. Range finding study was performed in duplicates prior to the initiation of study. Flasks were used as a test vessel. To each test flask were added test organism and a solution of test chemical. Two control vessels, one control with no test chemical but inoculated with T. pyriformis, and the other, a blank control, which has neither test chemical nor test organism were run simultaneously during the study. All test concentrations experiments were performed in duplicates. Test conditions allow for 8-9 cell cycles in control cultures. Only replicates with control-absorbency values > 0.6 but < 0.75 were used for the analysis. Population density of test organism was measured spectrophotometrically at 540 nm. The 50% growth inhibitory concentrations, IGC50, were determined by Probit Analysis of Statistical Analysis System (SAS) software. On the basis of the effect on growth inhibition of the test organism Tetrahymena pyriformis, the 40 hr IGC50 value was determined to be 204.64 mg/l.
Key value for chemical safety assessment
Additional information
Toxicity to microorganism study was performed using Tetrahymena pyriformis as a test organism (T. W. Schultz et. al., 1999 and authoritative database, 2012). The study was carried out under static conditions. Stock solutions of test chemical were prepared in dimethyl sulfoxide. Exact test chemical concentrations used for the study was not known, but each test replicate consists of six to eight different concentrations. Range finding study was performed in duplicates prior to the initiation of study. Flasks were used as a test vessel. To each test flask were added test organism and a solution of test chemical. Two control vessels, one control with no test chemical but inoculated with T. pyriformis, and the other, a blank control, which has neither test chemical nor test organism were run simultaneously during the study. All test concentrations experiments were performed in duplicates. Test conditions allow for 8-9 cell cycles in control cultures. Only replicates with control-absorbency values > 0.6 but < 0.75 were used for the analysis. Population density of test organism was measured spectrophotometrically at 540 nm. The 50% growth inhibitory concentrations, IGC50, were determined by Probit Analysis of Statistical Analysis System (SAS) software. On the basis of the effect on growth inhibition of the test organism Tetrahymena pyriformis, the 40 hr IGC50 value was determined to be 204.64 mg/l.
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