Decan-5-olide

Toxicity to microorganisms

Link to relevant study record(s)

Reference

Endpoint:

toxicity to microorganisms, other

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Data is from peer reviewed journal

Justification for type of information:

Data is from peer reviewed journal

Qualifier:

according to guideline

Guideline:

other: as mentioned below

Principles of method if other than guideline:

Toxicity to microorganism study was carried out.

GLP compliance:

not specified

Analytical monitoring:

not specified

Vehicle:

yes

Remarks:

dimethyl sulfoxide (DMSO) was used as a vehicle.

Test organisms (species):

Tetrahymena pyriformis

Test type:

static

Water media type:

freshwater

Total exposure duration:

40 h

Details on test conditions:

TEST SYSTEM
- Test vessel: Flasks were used as a test vessel.
- No. of vessels per concentration (replicates): Duplicates
- Other: Two control vessels, one control with no test chemical but inoculated with T. pyriformis, and the other, a blank control, which has neither test chemical nor test organism were run simultaneously during the study.

EFFECT PARAMETERS MEASURED (with observation intervals if applicable): Population density of test organism was measured spectrophotometrically at 540 nm.

TEST CONCENTRATIONS
- Range finding study: yes, range finding study was performed in duplicates prior to the initiation of study.

Reference substance (positive control):

not specified

Key result

Duration:

40 h

Dose descriptor:

other: IGC50

Effect conc.:

204.64 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth inhibition

Reported statistics and error estimates:

The 50% growth inhibitory concentrations, IGC50, were determined by Probit Analysis of Statistical Analysis System (SAS) software

Table: Toxicity of test chemical result.

 

Chemical	Log 1/IGC50	logKow (1-octanol/water)
Test chemical	-0.08	2.47

Validity criteria fulfilled:

not specified

Conclusions:

Based on effect on growth inhibition of the test organism Tetrahymena pyriformis, the 40 hr IGC50 value was determined to be 204.64 mg/l.

Executive summary:

Toxicity to microorganism study was performed using Tetrahymena pyriformis as a test organism. The study was carried out under static conditions. Stock solutions of test chemical were prepared in dimethyl sulfoxide. Exact test chemical concentrations used for the study was not known, but each test replicate consists of six to eight different concentrations. Range finding study was performed in duplicates prior to the initiation of study. Flasks were used as a test vessel. To each test flask were added test organism and a solution of test chemical. Two control vessels, one control with no test chemical but inoculated with T. pyriformis, and the other, a blank control, which has neither test chemical nor test organism were run simultaneously during the study. All test concentrations experiments were performed in duplicates. Test conditions allow for 8-9 cell cycles in control cultures. Only replicates with control-absorbency values > 0.6 but < 0.75 were used for the analysis. Population density of test organism was measured spectrophotometrically at 540 nm. The 50% growth inhibitory concentrations, IGC50, were determined by Probit Analysis of Statistical Analysis System (SAS) software. On the basis of the effect on growth inhibition of the test organism Tetrahymena pyriformis, the 40 hr IGC50 value was determined to be 204.64 mg/l.

Description of key information

Toxicity to microorganism study was performed using Tetrahymena pyriformis as a test organism (T. W. Schultz et. al., 1999 and authoritative database, 2012). The study was carried out under static conditions. Stock solutions of test chemical were prepared in dimethyl sulfoxide. Exact test chemical concentrations used for the study was not known, but each test replicate consists of six to eight different concentrations. Range finding study was performed in duplicates prior to the initiation of study. Flasks were used as a test vessel. To each test flask were added test organism and a solution of test chemical. Two control vessels, one control with no test chemical but inoculated with T. pyriformis, and the other, a blank control, which has neither test chemical nor test organism were run simultaneously during the study. All test concentrations experiments were performed in duplicates. Test conditions allow for 8-9 cell cycles in control cultures. Only replicates with control-absorbency values > 0.6 but < 0.75 were used for the analysis. Population density of test organism was measured spectrophotometrically at 540 nm. The 50% growth inhibitory concentrations, IGC50, were determined by Probit Analysis of Statistical Analysis System (SAS) software. On the basis of the effect on growth inhibition of the test organism Tetrahymena pyriformis, the 40 hr IGC50 value was determined to be 204.64 mg/l.

Additional information

Toxicity to microorganism study was performed using Tetrahymena pyriformis as a test organism (T. W. Schultz et. al., 1999 and authoritative database, 2012). The study was carried out under static conditions. Stock solutions of test chemical were prepared in dimethyl sulfoxide. Exact test chemical concentrations used for the study was not known, but each test replicate consists of six to eight different concentrations. Range finding study was performed in duplicates prior to the initiation of study. Flasks were used as a test vessel. To each test flask were added test organism and a solution of test chemical. Two control vessels, one control with no test chemical but inoculated with T. pyriformis, and the other, a blank control, which has neither test chemical nor test organism were run simultaneously during the study. All test concentrations experiments were performed in duplicates. Test conditions allow for 8-9 cell cycles in control cultures. Only replicates with control-absorbency values > 0.6 but < 0.75 were used for the analysis. Population density of test organism was measured spectrophotometrically at 540 nm. The 50% growth inhibitory concentrations, IGC50, were determined by Probit Analysis of Statistical Analysis System (SAS) software. On the basis of the effect on growth inhibition of the test organism Tetrahymena pyriformis, the 40 hr IGC50 value was determined to be 204.64 mg/l.