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Ecotoxicological information

Short-term toxicity to fish

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Reference
Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study
Justification for type of information:
Experimental test result performed according to the guideline.
Qualifier:
according to
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
Principles of method if other than guideline:
Fish Acute Toxicity test according to OECD Guideline 203 was conducted for test material
GLP compliance:
not specified
Analytical monitoring:
no
Vehicle:
not specified
Details on test solutions:
The test substance was soluble in water. Therefore, the stock solution was prepared by dissolving 2 g of the test substance in 2 liters of potable water (passed through reverse osmosis system) with continuous 48 hours stirring, after stirring the stock was analytically detected and the concentration was found to be 168.12 mg/L. On thebasis of this concentration further test concentrations prepare for achieving test concentrations of 6.25 mg/L,12.5 mg/L,25mg/L,50 mg/L,100 mg/L ,respectively.
Test organisms (species):
Danio rerio (previous name: Brachydanio rerio)
Details on test organisms:
TEST ORGANISM
- Common name:Zebra Fish
- Strain:Danio rerio
- Source: Unique Aqua Fish Corner
- Length at study initiation (length definition, mean, range and SD): average 1.88 cm
- Weight at study initiation (mean and range, SD): average 0.0792 g
-Housing:The fishes were kept in a static tank in tap water passed through reverse osmosis system, under natural conditions along with proper feed and aeration.
-Duration of housing: 15 days
-Photoperiod: 16 hours light and 8 hours dark
-Diet : Standard brand feed, once daily
-Acclimatization: seven days
-feeding frequency: Once in a day
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h
Hardness:
200 mg of CaCO3
Test temperature:
23 °C
pH:
6.9
Nominal and measured concentrations:
6.25mg/L,12.5mg/L,25mg/L,50mg/L,100mg/L
Details on test conditions:
TEST SYSTEM - Test vessel: Plastic aquaria
- Material, size, headspace, fill volume: 5 liters of plastic aquaria filled with 2 liter of water
- Aeration: Aeration in test vessels was provided 1 day before the start of experiment
- No. of organisms per vessel: 8 fishes OTHER TEST CONDITIONS
- Photoperiod: 16 hours light and 8 hours dark
Reference substance (positive control):
not specified
Key result
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
6.25 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Duration:
96 h
Dose descriptor:
LC100
Effect conc.:
12.5 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality

9.1. Mortality and symptoms

Nominal Concentration

Number of fish

Mortality (cumulated) after

 

 

(mg/l)

 

3 h

6 h

24h

48h

72h

96h

 

 

V1

V1

V1

V1

V1

V1

Control

8

-

-

-

-

-

-

6.25

8

-

-

-

-

-

2

12.5

8

-

-

4

4

4

4

25

8

-

-

4

4

4

4

50

8

4

4

4

4

4

4

100

8

4

4

4

4

4

4

Visible Symptoms:

 

 

 

 

Nominal Concentration

Number of fish

3 h

6 h

24h

48h

72h

96h

 

 

 

 

 

 

(mg/l)

 

V1

V1

V1

V1

V1

V1

 

 

Control

8

NS4

NS4

NS4

NS4

NS4

NS4

6.25

8

NS4

NS4

NS4

NS4

NS4

NS2

12.5

8

IS4

IS4

-

-

-

-

25

8

IS4

IS4

-

-

-

-

50

8

-

-

-

-

-

-

100

8

-

-

-

-

-

-

Explanation of symptoms:

A: apathy

N: Narcotic-like state

C: Swimming near water surface

M: Slow movements compared to control

NS: No abnormal symptoms

S: Surfacing behaviour

L: Loss of equilibrium

SM: Shimming movement

H1: Hyperactivity

H2: Hypo activity

B: Bending behavior

F: Fin rot

IS: Invert Swimming

SB: Swimming behaviour

RF: Respiratory Function

P: Pigmentation

8.2. pH and oxygen content:

Nominal

Number of fish

pH readings after

 

 

 

24h

48h

72h

96h

Concentration

 

(mg/l)

 

 

 

 

 

 

 

V1

V1

V1

V1

Control

8

7.3

7.4

7.5

7.6

6.25

8

7.5

7.6

7.8

8.0

12.5

8

-

-

-

-

25

8

-

-

-

-

50

8

-

-

-

-

100

8

-

-

-

-

Nominal

Number of

Oxygen content (mg/L) after

 

 

 

Concentrationfish

 

24h

 

48h

 

72h

 

96h

 

(mg/l)

 

 

V1

V2

V1

V2

V1

V2

V1

V2

Control

4

4

6.9

6.9

6.8

6.8

6.7

6.7

6.6

6.6

6.25

4

4

6.8

6.8

6.6

6.6

6.4

6.4

6.3

6.3

12.5

4

4

-

-

-

-

-

-

-

-

25

4

4

-

-

-

-

-

-

-

-

50

4

4

-

-

-

-

-

-

-

-

100

4

4

-

-

-

-

-

-

-

-

8.3. Test temperature

 

 

 

 

 

 

 

 

Nominal

Number of

Temperature (°C) after

 

 

 

 

Concentrationfish

 

24h

 

48h

 

72h

 

96h

 

(mg/l)

 

 

V1

V2

V1

V2

V1

V2

V1

V2

Control

4

4

23.7

23.7

23.6

23.6

23.8

23.8

24.1

24.1

6.25

4

4

23.7

23.7

23.6

23.6

23.8

23.8

24.1

24.1

12.5

4

4

-

-

-

-

-

-

-

-

25

4

4

-

-

-

-

-

-

-

-

50

4

4

-

-

-

-

-

-

-

-

100

4

4

-

-

-

-

-

-

-

-

Validity criteria fulfilled:
yes
Conclusions:
Based on nominal concentrations, experimental median lethal Concentrations [LC-50 (96 h)] for test material on Zebra Fish Danio rerio was determine to be 6.25 mg/l , LC 100 (96 hours) (lowest loading at which 100% mortality was observed) = 12.5 mg/L
Executive summary:

Study was conducted to access the effect of test chemical on the growth of fish Danio rerio. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test). The test substance was soluble in water. Therefore, the stock solution was prepared by dissolving 2 g of the test substance in 2 liters of potable water (passed through reverse osmosis system) with continuous 48 hours stirring, after stirring the stock was analytically detected and the concentration was found to be 168.12 mg/L. On the basis of this concentration further test concentrations prepare for achieving test concentrations of 6.25 mg/L,12.5 mg/L,25mg/L,50 mg/L,100 mg/L ,respectively.

Bowl aquaria containing 2 liters of potable water (passed through reverse osmosis system) were loaded with 8 fishes. A static procedure was used for the study and it was conducted in compliance with the OECD guideline 203. After 96 hours of exposure to test material to various nominal test concentrations, LC50 was determine to be 6.25 mg/l . Based on the LC50, it can be consider that the chemical was test material and can be consider to be classified as aquatic chronic 2 as per the CLP classification criteria.

Description of key information

Short-term toxicity to fish:

Study was conducted to access the effect of test chemical on the growth of fish Danio rerio. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test).The test substance was soluble in water. Therefore, the stock solution was prepared by dissolving 2 g of the test substance in 2 liters of potable water (passed through reverse osmosis system) with continuous 48 hours stirring, after stirring the stock was analytically detected and the concentration was found to be 168.12 mg/L. On the basis of this concentration further test concentrations prepare for achieving test concentrations of 6.25 mg/L,12.5 mg/L,25mg/L,50 mg/L,100 mg/L ,respectively.

Bowl aquaria containing 2 liters of potable water (passed through reverse osmosis system) were loaded with 8 fishes. A static procedure was used for the study and it was conducted in compliance with the OECD guideline 203. After 96 hours of exposure to test material to various nominal test concentrations, LC50 was determine to be 6.25 mg/l . Based on the LC50, it can be consider that the chemical was test material and can be consider to be classified as aquatic chronic 2 as per the CLP classification criteria.

Key value for chemical safety assessment

LC50 for freshwater fish:
6.25 mg/L

Additional information

Short-term toxicity to fish:

Study was conducted to access the effect of test chemical on the growth of fish Danio rerio. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test).The test substance was soluble in water. Therefore, the stock solution was prepared by dissolving 2 g of the test substance in 2 liters of potable water (passed through reverse osmosis system) with continuous 48 hours stirring, after stirring the stock was analytically detected and the concentration was found to be 168.12 mg/L. On the basis of this concentration further test concentrations prepare for achieving test concentrations of 6.25 mg/L,12.5 mg/L,25mg/L,50 mg/L,100 mg/L ,respectively.

Bowl aquaria containing 2 liters of potable water (passed through reverse osmosis system) were loaded with 8 fishes. A static procedure was used for the study and it was conducted in compliance with the OECD guideline 203. After 96 hours of exposure to test material to various nominal test concentrations, LC50 was determine to be 6.25 mg/l . Based on the LC50, it can be consider that the chemical was test material and can be consider to be classified as aquatic chronic 2 as per the CLP classification criteria.