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Ecotoxicological information

Endpoint summary

Administrative data

Description of key information

Short-term toxicity to fish:

Study was conducted to access the effect of test chemical on the growth of fish Danio rerio. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test).The test substance was soluble in water. Therefore, the stock solution was prepared by dissolving 2 g of the test substance in 2 liters of potable water (passed through reverse osmosis system) with continuous 48 hours stirring, after stirring the stock was analytically detected and the concentration was found to be 168.12 mg/L. On the basis of this concentration further test concentrations prepare for achieving test concentrations of 6.25 mg/L,12.5 mg/L,25mg/L,50 mg/L,100 mg/L ,respectively.

Bowl aquaria containing 2 liters of potable water (passed through reverse osmosis system) were loaded with 8 fishes. A static procedure was used for the study and it was conducted in compliance with the OECD guideline 203. After 96 hours of exposure to test material to various nominal test concentrations, LC50 was determine to be 6.25 mg/l . Based on the LC50, it can be consider that the chemical was test material and can be consider to be classified as aquatic chronic 2 as per the CLP classification criteria.

Long term toxicity to fish:

On the basis of EPI suite, ECOSAR version 1.1, the NOEC value for long term toxicity to aquatic invertebrates was predicted to be 4.5 mg/l for test material in 14 days.  Based on this value it can be concluded that the substance is considered to be not toxic to aquatic environment and cannot be classified   as per the criteria mentioned in CLP regulation.

Short-term toxicity to aquatic invertebrates:

Aim of this study was to assess the short term toxicity of test material to aquatic invertebrates daphnia magna. Study was performed according to the OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test) in a static system for the total exposure period of 48 hrs.

 

The stock solution 100 mg/l was prepared by dissolving colourless liquid in reconstituted water. Test solutions of required concentration as were prepared by mixing the stock solution of the test sample with reconstituted test water. It was not possible to test at higher concentration because of limited solubility of tested sample (Changable sentence according to the chemical). 5, 10, 20, 40 and 80 mg/l concentrations were used in the study. Effects on immobilisation were observed for 48 hours. With the test substance one positive control Potassium dichromate (K2Cr2O7) was also run simultaneously. After the exposure of chemical, effect concentration EC50 was calculated using nonlinear regression by the software Prism 4.0.

  The median effective concentration (EC50) for the test substance , in Daphnia magna was determined to be 30.1 mg/L on the basis of mobility inhibition effects in a 48 hour study. Based on the EC50 value, substance is likely to be hazardous to aquatic invertebrate and can be classified as aquatic chronic 3 category as per the CLP classification criteria.

Long term toxicity to aquatic invertebrate:

On the basis of EPI suite, ECOSAR version 1.1, the NOEC value for long term toxicity to aquatic invertebrates was predicted to be 2.90 mg/l for test material in 21 days.  Based on this value it can be concluded that the substance is considered to be not toxic to aquatic environment and can not be classified  as per the criteria mentioned in CLP regulation.

Toxicity to aquatic algae and cyanobacteria:

Aim of this study was to evaluate the nature of chemical test chemical when comes in contact with the test organism Desmodesmus subspicatus (previous name: Scenedesmus subspicatus). Test was conducted according to the OECD guideline 201. The stock solution 100 mg/l was prepared by dissolving colourless liquid in OECD growth medium. Test solutions of required concentrations were prepared by mixing the stock solution of the test sample with OECD growth medium and inoculum culture.2.5, 5, 10, 20, 40 and 80 mg/lnominal concentrations were used.

With the test substance one positive control Potassium dichromate (K2Cr2O7) was also run simultaneously. After the exposure of chemical, effect concentration EC50 was calculated using nonlinear regression by the software Prism 4.0. Effect on the growth of algae was determine after an exposure period of 72 hrs.

The median effective concentration (EC50) for the test substance delta-decalactone, in algae was determined to be 20.5 mg/L on the basis of growth rate inhibition effects in a 72 hour study. Based on the EC50 value, which indicates that the substance is likely to be hazardous to aquatic algae and can be classified as aquatic chronic 3 category as per the CLP classification criteria.

Toxicity to microorganisms:

Toxicity to microorganisms was examined on tetrahymena pyriformis for 40 hours by static population growth impairment assay. Population density measured spectrophotometrically at 540 nm. Value found to be log(1/IC50)=-0.08 for IC50 in mM.

Additional information

Short-term toxicity to fish:

Study was conducted to access the effect of test chemical on the growth of fish Danio rerio. Test conducted according to OECD Guideline 203 (Fish, Acute Toxicity Test).The test substance was soluble in water. Therefore, the stock solution was prepared by dissolving 2 g of the test substance in 2 liters of potable water (passed through reverse osmosis system) with continuous 48 hours stirring, after stirring the stock was analytically detected and the concentration was found to be 168.12 mg/L. On the basis of this concentration further test concentrations prepare for achieving test concentrations of 6.25 mg/L,12.5 mg/L,25mg/L,50 mg/L,100 mg/L ,respectively.

Bowl aquaria containing 2 liters of potable water (passed through reverse osmosis system) were loaded with 8 fishes. A static procedure was used for the study and it was conducted in compliance with the OECD guideline 203. After 96 hours of exposure to test material to various nominal test concentrations, LC50 was determine to be 6.25 mg/l . Based on the LC50, it can be consider that the chemical was test material and can be consider to be classified as aquatic chronic 2 as per the CLP classification criteria.

Long term toxicity to fish:

On the basis of EPI suite, ECOSAR version 1.1, the NOEC value for long term toxicity to aquatic invertebrates was predicted to be 4.5 mg/l for test material in 14 days.  Based on this value it can be concluded that the substance is considered to be not toxic to aquatic environment and cannot be classified   as per the criteria mentioned in CLP regulation.

Short-term toxicity to aquatic invertebrates:

Aim of this study was to assess the short term toxicity of test material to aquatic invertebrates daphnia magna. Study was performed according to the OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test) in a static system for the total exposure period of 48 hrs.

 

The stock solution 100 mg/l was prepared by dissolving colourless liquid in reconstituted water. Test solutions of required concentration as were prepared by mixing the stock solution of the test sample with reconstituted test water. It was not possible to test at higher concentration because of limited solubility of tested sample (Changable sentence according to the chemical). 5, 10, 20, 40 and 80 mg/l concentrations were used in the study. Effects on immobilisation were observed for 48 hours. With the test substance one positive control Potassium dichromate (K2Cr2O7) was also run simultaneously. After the exposure of chemical, effect concentration EC50 was calculated using nonlinear regression by the software Prism 4.0.

  The median effective concentration (EC50) for the test substance , in Daphnia magna was determined to be 30.1 mg/L on the basis of mobility inhibition effects in a 48 hour study. Based on the EC50 value, substance is likely to be hazardous to aquatic invertebrate and can be classified as aquatic chronic 3 category as per the CLP classification criteria.

Long term toxicity to aquatic invertebrate:

On the basis of EPI suite, ECOSAR version 1.1, the NOEC value for long term toxicity to aquatic invertebrates was predicted to be 2.90 mg/l for test material in 21 days.  Based on this value it can be concluded that the substance is considered to be not toxic to aquatic environment and can not be classified  as per the criteria mentioned in CLP regulation.

Toxicity to aquatic algae and cyanobacteria:

Aim of this study was to evaluate the nature of chemical test chemical when comes in contact with the test organism Desmodesmus subspicatus (previous name: Scenedesmus subspicatus). Test was conducted according to the OECD guideline 201. The stock solution 100 mg/l was prepared by dissolving colourless liquid in OECD growth medium. Test solutions of required concentrations were prepared by mixing the stock solution of the test sample with OECD growth medium and inoculum culture.2.5, 5, 10, 20, 40 and 80 mg/lnominal concentrations were used.

With the test substance one positive control Potassium dichromate (K2Cr2O7) was also run simultaneously. After the exposure of chemical, effect concentration EC50 was calculated using nonlinear regression by the software Prism 4.0. Effect on the growth of algae was determine after an exposure period of 72 hrs.

The median effective concentration (EC50) for the test substance delta-decalactone, in algae was determined to be 20.5 mg/L on the basis of growth rate inhibition effects in a 72 hour study. Based on the EC50 value, which indicates that the substance is likely to be hazardous to aquatic algae and can be classified as aquatic chronic 3 category as per the CLP classification criteria.

Toxicity to microorganisms:

Toxicity to microorganisms was examined on tetrahymena pyriformis for 40 hours by static population growth impairment assay. Population density measured spectrophotometrically at 540 nm. Value found to be log(1/IC50)=-0.08 for IC50 in mM.