Registration Dossier

Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
February 01 - February 03, 1995
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: done under GLP and OECD method

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1995
Report Date:
1995

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
EU Method C.2 (Acute Toxicity for Daphnia)
Version / remarks:
and OECD nr. 202 part 1
GLP compliance:
yes

Test material

Reference
Name:
Unnamed
Type:
Constituent

Sampling and analysis

Analytical monitoring:
yes
Details on sampling:
samples (50 ml in duplicate) taken from control and each exposure groups (replicates pooled) at 0 hours and 48 hours and analyzed.

Test solutions

Vehicle:
yes
Details on test solutions:
2.2, 4.6, 10, 22, 46, 100, 220mg/l (nominal) solutions of A-87440.0 in Elendt M7 medium

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Common name: Daphnia magna
- Strain: Straus
- Source: IRChA, France

Study design

Test type:
semi-static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h
Remarks on exposure duration:
Evaluations at 24hr and 48hr

Test conditions

Test temperature:
20°C ± 1°C
pH:
7.3 - 7.5
Dissolved oxygen:
8.6 - 8.7mg/L
Nominal and measured concentrations:
Nominal: 2.2, 4.6, 10, 22, 46, 100, 220mg/L
Measured: 2.1, 4.2, 9.1, 20, 43, 93, 210mg/L
Details on test conditions:
TEST SYSTEM
- Test vessel: Glass jars
- Type: loosely covered with aluminium foil to help reduce evaporation.
- fill volume: 200 ml
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 2

OTHER TEST CONDITIONS
- Photoperiod: 16hr light, 8hr dark

Results and discussion

Effect concentrationsopen allclose all
Duration:
24 h
Dose descriptor:
EC50
Effect conc.:
110 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: 86 - 130 (95% CL)
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
44 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: 33 - 58 (95% CL)
Duration:
48 h
Dose descriptor:
NOEC
Effect conc.:
9.1 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
test mat.
Basis for effect:
mobility
Details on results:
HIghest conc resulting in 0% immobilisation: 9.1mg/l
Lowest conc resulting in 100% immobilisation: 210mg/l

Any other information on results incl. tables

All results expressed in terms of measured concentrations. Measured concentrations ranged from 90 -95% of nominal at 0 hours and 89 -102% of nominal at 48 hours.

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
The 48hr EC 50 (immobilisation) value for A-87440.0 with Daphnia Magna is 44mg/l