5-thiazolylmethanol

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

key study

Study period:

03 Feb - 06 Feb, 1995

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Done under GLP and OECD methods

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1995

Report date:

1995

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

EU Method C.3 (Algal Inhibition test)

Version / remarks:

Method C3, Directive 92/69/EEC

Test guideline 2

Qualifier:

according to guideline

Guideline:

OECD Guideline 201 (Alga, Growth Inhibition Test)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

5-thiazolylmethanol

EC Number:

414-780-9

EC Name:

5-thiazolylmethanol

Cas Number:

38585-74-9

Molecular formula:

C4H5NOS

IUPAC Name:

(1,3-thiazol-5-yl)methanol

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

tes concnetrations verified by HPLC. water samples ( unfiltered) were taken from control and treatment groups at 0 and 72 hour

Test solutions

Vehicle:

no

Details on test solutions:

The test substance was dispersed directly into sterile nutrient medium to produce a solution twice the concentration of the intended dose.

Test organisms

Test organisms (species):

Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)

Details on test organisms:

TEST ORGANISM
- Strain: CCAP 278/4
- Source: Culture Centre of Algae & Protozoa c/o Freshwater Biological Association, Cumbria, UK.

Study design

Test type:

semi-static

Water media type:

freshwater

Total exposure duration:

72 h

Test conditions

Test temperature:

23±2°C

Nominal and measured concentrations:

Nominal conc: 100mg/l
Actual measured conc. 96mg/l
all results based on measured concentration. Values ranged from 95-96% of nominal at 0 hours and 96-98% at 72 hours.

Details on test conditions:

Orbital shaker at 120 cycles/min
72hrs under continuos illumination of approx. 7,000 lux.

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

> 96 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

growth rate

Effect concentrations 2

Duration:

72 h

Dose descriptor:

NOEC

Effect conc.:

>= 96 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

biomass

Effect concentrations 3

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

> 96 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

biomass

Details on results:

Starting from the real concentrations of exposure:
ECb50(0-72h): >96mg/l
No-observed effect level: ≥ 96mgml

Any other information on results incl. tables

Samples taken at 0, 24, 48 and 72hr and cell densities were determined by direct counting with aid of a Coulter®Multisizer II particle counter.

Analyses of product solution concentrations was by HPLC.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The 0-72hr ErC50 of test substance was calculated to be >96 mg/l

The 72hr EbC50 of test substance was calculated to be >96 mg/l

No-observed effect level: ≥96mg/l

Executive summary:

A-87440.0 is not inhibitory to the growth of Selenastrum capricornutum, strain no. CCAP 278/4 at concentrations of up to 96mg/l