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Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
experimental study
Adequacy of study:
key study
Study period:
03 Feb - 06 Feb, 1995
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: Done under GLP and OECD methods

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1995
Report Date:
1995

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to
Guideline:
EU Method C.3 (Algal Inhibition test)
Version / remarks:
Method C3, Directive 92/69/EEC
Qualifier:
according to
Guideline:
OECD Guideline 201 (Alga, Growth Inhibition Test)
GLP compliance:
yes

Test material

Reference
Name:
Unnamed
Type:
Constituent

Sampling and analysis

Analytical monitoring:
yes
Details on sampling:
tes concnetrations verified by HPLC. water samples ( unfiltered) were taken from control and treatment groups at 0 and 72 hour

Test solutions

Vehicle:
no
Details on test solutions:
The test substance was dispersed directly into sterile nutrient medium to produce a solution twice the concentration of the intended dose.

Test organisms

Test organisms (species):
Pseudokirchneriella subcapitata (previous names: Raphidocelis subcapitata, Selenastrum capricornutum)
Details on test organisms:
TEST ORGANISM
- Strain: CCAP 278/4
- Source: Culture Centre of Algae & Protozoa c/o Freshwater Biological Association, Cumbria, UK.

Study design

Test type:
semi-static
Water media type:
freshwater
Total exposure duration:
72 h

Test conditions

Test temperature:
23±2°C
Nominal and measured concentrations:
Nominal conc: 100mg/l
Actual measured conc. 96mg/l
all results based on measured concentration. Values ranged from 95-96% of nominal at 0 hours and 96-98% at 72 hours.
Details on test conditions:
Orbital shaker at 120 cycles/min
72hrs under continuos illumination of approx. 7,000 lux.

Results and discussion

Effect concentrationsopen allclose all
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
> 96 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
test mat.
Basis for effect:
growth rate
Duration:
72 h
Dose descriptor:
NOEC
Effect conc.:
>= 96 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
test mat.
Basis for effect:
biomass
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
> 96 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
test mat.
Basis for effect:
biomass
Details on results:
Starting from the real concentrations of exposure:
ECb50(0-72h): >96mg/l
No-observed effect level: ≥ 96mgml

Any other information on results incl. tables

Samples taken at 0, 24, 48 and 72hr and cell densities were determined by direct counting with aid of a Coulter®Multisizer II particle counter.

Analyses of product solution concentrations was by HPLC.

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
The 0-72hr ErC50 of test substance was calculated to be >96 mg/l

The 72hr EbC50 of test substance was calculated to be >96 mg/l

No-observed effect level: ≥96mg/l
Executive summary:

A-87440.0 is not inhibitory to the growth of Selenastrum capricornutum, strain no. CCAP 278/4 at concentrations of up to 96mg/l