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EC number: 258-436-4
CAS number: 53220-22-7
Concentration of the test item both in the
test and control group were below the quantification limit (LOQ = 5
mg/L) considering tenfold concentration of the samples before the
analysis. Therefore, nominal concentrations were used.
Growth inhibition: Statistical comparisons
of average specific growth rates and yield in control and in treated
groups were carried out using 2 Sample t-Test (a= 0.05) by TOXSTAT
software. The results of the statistical evaluation showed that the
0-72 h average specific growth rate and yield at the treatmentgroup
(100 % v/v saturated solution, equivalent to 100 mg/L nominal
concentration) was not statistically significantly different from the
untreated control. Accordingly, the No Observed Effect Concentration
(NOEC) was determinedas the saturation concentration (equivalent to
100 mg/L nominal concentration). The 72 h-EC50 values based on growth
rate and yield were determined to be higher than saturation.
Shape of the algal cells: Abnormal
appearance of the algal cells was not observed during the experiment.
Cell Density in Control: The cell density in
the control has increased from nominal N = 1 × 104 cells/mL at the start
of the test (0 hours) to N = 64.17 × 104 cells/mL (mean value) after 72
hours of exposure. Thus, the algal growth in the control was high enough
to pass the validity criteria in this assay.
This 72-h algal growth inhibition test with
Pseudokirchneriella subcapitata was performed in accordance with OECD
no. 201 guideline, EU method C.3 and OPPTS 850.5400 guideline. The
obtained results showed that the test item ditetradecyl
peroxydicarbonate had no toxic effect at aquatic saturation (i.e. 100%
v/v saturated solution, equivalent to 100 mg/L nominal concentration) on
the algae. Accordingly, the EC10, EC50 and the LOEC values based on
growth rate and yield were determined to be higher than the solubility
level of the test item in the test medium which corresponds to a nominal
value of > 100 mg/L. The NOEC was determined to be 100 mg/L (nominal
In a 72-h algal growth inhibition
test with Pseudokirchneriella subcapitata the EC50 and the NOEC (both
based on growth rate and yield) were determined to be > 100 mg/L and
100 mg/L, respectively.
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