Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 258-436-4
CAS number: 53220-22-7
Ditetradecyl peroxydicarbonate was tested in
an acute toxicity test with Poecilia reticulata (guppy) under
semi-static conditions in accordance with EU method C.1 and OECD
guideline No. 203. The test was performed in duplicate. The fish
survived for four days in a saturated solution which contained 1000 mg
of the test substance/L. Binormal theory dicates that when 10 fish are
used showing 0 % mortality, there is a 99.9 % confidence that the LC50
(96 h) is greater than 1000 mg/L. However, given that during the test
flakes of the test substance remained visible at the bottom of the
vessel, the LC50 value should be handled with care. No deviations in the
behaviour or appearance of the fish were observed.
The LC50 (96 h) of ditetradecyl peroxydicarbonate is greater than 1000 mg/L.
Key acute fish study
Ditetradecyl peroxydicarbonate was tested in an acute toxicity test with Poecilia reticulata (guppy) under semi-static conditions in accordance with EU method C.1 and OECD guideline No. 203. The test was performed in duplicate. The fish survived for four days in a saturated solution which contained 1000 mg of the test substance/L. Binormal theory dicates that when 10 fish are used showing 0 % mortality, there is a 99.9 % confidence that the LC50 (96 h) is greater than 1000 mg/L. However, given that during the test flakes of the test substance remained visible at the bottom of the vessel, the LC50 value should be handled with care. No deviations in the behaviour or appearance of the fish were observed.
Supporting FET study with the test item and structural analogue substance (CAS 26322-14-5)
The objective of this study was to screen the effects of the test item and its structural analogue substance (CAS 26322-14-5) for its effects on newly fertilized zebra fish eggs and hatchlings over an exposure period of 96 hours according to a simplified OECD 236 guideline. The intention was to demonstrate a similar range of effects in support of a read-across approach. Both, the target and the source substance are extremely poorly soluble, with solubility limits under or at the limits of the available analytical methods available. A 72 hour slow stir technique was therefore used to allow an extended period for the parent substances or resulting degradation products to equilibrate at their corresponding solubility limits. Such a water accommodated fraction approach is preferentially supported with chemical analysis in order to demonstrate exposure to the materials concerned. For the materials tested this was not possible. For this reason a daily refreshment regime was chosen to ensure a worst case exposure. Testing was otherwise conducted according to the OECD 236 guideline with daily observations with the modifications as indicated under test guideline modifications and deviations. Every 24 hours, observations were recorded. All atypical effects on the embryo in comparison to the controls were noted. At the end of the exposure period, acute toxicity was determined based on a positive outcome in any of the four apical observations as detailed in the OECD 236 guideline. The LC50 was then estimated where possible. For other observations there is as yet no finalized guidance on how to interpret any non-lethal findings from this assay. Other nonlethal findings were therefore recorded only at this stage. Previous work using the OECD 236 guideline for predicting the effects of organic peroxides on adult fish showed a good level of concordance with existing adult fish data. It was noted however during this work that, in order to provide a sufficiently conservative estimation of an adult fish LC50 non-lethal effects should ideally also be included in the prediction. For this reason fish considered to have severe malformations that would ultimately result in their death were considered dead in order to make the LC50 prediction for adult fish as worst case as possible. Acute toxicity is usually expressed as EC20,50,80 (Effect Concentration) values. The ECn values are the concentrations of the test substance showing n% reduction in survival relative to the controls. Depending on the test results obtained, the LOEC (Lowest Observed Effect Concentration) and NOEC (No Observed Effect Concentration) can also be determined. The LOEC is defined as the lowest tested concentration which survival is significantly reduced compared to the control. The NOEC is defined as the highest tested concentration at which survival shows no significant difference relative to the control. Endpoints are usually calculated using a validated statistical software program using the William’s and Trimmed Spearman-Karber / probit methods as appropriate. Dependent on the data generated statistical calculations cannot usually be carried out in screening studies due to the limited concentration range, in which case an estimation of the endpoint range has been made. In the event that the test substance is very poorly soluble, instable or a mixture, loading concentrations; Effect loading concentration (ELn) and No observed effect loading rate (NOELR) were used to express the toxicity. The similar results observed when fish embryos and hatchlings were exposed to both substances is considered to support similar acute toxicity of both materials to adult Fish species. Read-across of the existing acute fish data is therefore considered justified. In addition the hydrolysis of both parent materials is also expected to occur in a similar way to Tetra and hexadecanol. Both are also of similar structure and limited solubility. The benefit of a WAF approach is such that test organisms are exposed to the soluble fractions of both parent and hydrolysis products. Work is ongoing to attempt quantification of Parent and /or degradation products to allow a better quantitative assessment of the effects.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
Welcome to the ECHA website. This site is not fully supported in Internet Explorer 7 (and earlier versions). Please upgrade your Internet Explorer to a newer version.
Do not show this message again