Dibenzyldimethylammonium chloride

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2019

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 201 (Freshwater Alga and Cyanobacteria, Growth Inhibition Test)

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

Dibenzyldimethylammonium chloride

EC Number:

202-903-7

EC Name:

Dibenzyldimethylammonium chloride

Cas Number:

100-94-7

Molecular formula:

C16H20N.Cl

IUPAC Name:

dibenzyldimethylammonium chloride

Test material form:

solid: crystalline

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

Analysis of the test media samples was conducted on fresh media at 0 hours and on corresponding old media at 72 hours. Measured test concentrations were determined from samples of test solution from each treatment and control group.

Test solutions

Vehicle:

no

Details on test solutions:

The test concentration was prepared by dissolving the appropriate weight of test
substance in 1000 mL of OECD medium by short agitation. Following preparation, all the test solutions were checked for the tyndall effect using
a Hach 2100 N turbidity meterr. The turbidity meter confirmed that there was no dispersed test substance present and therefore the test solution was used to prepare the remaining test solutions

Test organisms

Test organisms (species):

Pseudokirchneriella subcapitata (previous names: Raphidocelis subcapitata, Selenastrum capricornutum)

Details on test organisms:

The test organism, Raphidocelis subcapitata (Strain 278/4; formerly known as
Pseudokirchneriella subcapitata), was originally obtained from the Culture Collection
of Algae and Protozoa (CCAP), Oban, UK and is a representative species of the freshwater aquatic phytoplankton.

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

72 h

Test conditions

Hardness:

Hardness (mg CaCO3/L): 31

Test temperature:

Temperature Range (°C)
22.1 – 23.0°C

pH:

7.90 – 7.97

Salinity:

Alkalinity (mg CaCO3/L): 32

Conductivity:

168 -198 μS/cm

Nominal and measured concentrations:

The measured test substance concentrations were between 93 – 98 % of nominal test substance concentrations at 0 hours and 88 - 91 % of nominal concentrations at 72 hours.

Results and discussion

Effect concentrations

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

30 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Applicant's summary and conclusion

Validity criteria fulfilled:

yes