Registration Dossier

Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2019

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
GLP compliance:
yes (incl. certificate)

Test material

Reference
Name:
Unnamed
Type:
Constituent
Test material form:
solid: crystalline

Sampling and analysis

Analytical monitoring:
yes
Details on sampling:
Analysis of the test media samples was conducted on fresh media at 0 hours and on
corresponding old media at 48 hours

Test solutions

Vehicle:
no
Details on test solutions:
The definitive test was conducted at the following
nominal test substance concentrations: 10, 18, 32, 56 and 100 mg/L. Tons. A control treatment was also included in the test.

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
Juvenile Daphnia magna (less than 24 hours old) were taken from healthy laboratory
cultures maintained in media of the same composition and quality as that used in the
toxicity tests. These cultures were setup originally from dormant eggs (ephippia)
supplied byMicroBioTests Inc., Belgium.

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h

Test conditions

Hardness:
Hardness (mg /L as CaCO3) 252
Test temperature:
18.5– 20.1°C
pH:
7.80 - 7.97
Dissolved oxygen:
93.6 - 98.6%
Salinity:
Alkalinity (mg /L as CaCO3) 72
Conductivity:
584 - 612 (μS/cm)
Nominal and measured concentrations:
The measured test substance concentrations were between 87% – 99 % of nominal
test substance concentrations at 0 hours and 89% - 96 % of nominal concentrations
at 48 hours.

Results and discussion

Effect concentrations
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
30 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility

Applicant's summary and conclusion

Validity criteria fulfilled:
yes