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EC number: 202-903-7 | CAS number: 100-94-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Endpoint summary
Administrative data
Description of key information
Oral: An LD50 of ca. 1815 mg/kg was determined after an oral administration of the test substance to rats via gavage.
Inhalation: In an IHT test no mortalities were observed when rats were exposed for 8 hours to an atmosphere enriched with the test substance vapour generated at 20°C (13.75 mg/L) and 100°C (43.36 mg/L).
Dermal: No information available.
Key value for chemical safety assessment
Acute toxicity: via oral route
Link to relevant study records
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: no guideline was followed, observation period was only 7 days, acceptable for assessment
- Principles of method if other than guideline:
- Method: BASF test
- GLP compliance:
- no
- Remarks:
- GLP was not compulsatory at this time
- Limit test:
- no
- Specific details on test material used for the study:
- - appearance: liquid
- content: 50% in water - Species:
- rat
- Strain:
- not specified
- Remarks:
- Gassner
- Sex:
- male/female
- Route of administration:
- oral: gavage
- Vehicle:
- water
- Doses:
- Doses: 8000, 6400, 5000, 4000, 3200, 2500 cmm/kg.
Concentration: 30% (v/v) aqueous solution - No. of animals per sex per dose:
- 10
- Control animals:
- no
- Details on study design:
- - Duration of observation period following administration: 7 days.
- Frequency of observations and weighing: not specified
- Necropsy of survivors performed: yes.
- Other examinations performed: clinical signs, body weights. - Sex:
- male/female
- Dose descriptor:
- LD50
- Effect level:
- ca. 3 300 other: mm3/kg
- Remarks on result:
- other: worst case assumption: equivalent to LD50 = 1815 mg/kg considering the substance density and the 50% content of the substance in the test material
- Mortality:
- See table 1.
All animals exposed to 5000 cmm/kg and above deceased within 15 minutes. Surviving animals showed accelerated breathing and hunched position 2 hours after application, but no adverse effects were observed at day 3 and thereafter. - Clinical signs:
- Directly after application accelerated breathing, tremors, apathy, abdominal position and clonic convulsions were observed.
- Body weight:
- Mean body weights wre determined to be 241.2 g for males and 167.1 g for females.
- Gross pathology:
- venous congestion
- Interpretation of results:
- Category 4 based on GHS criteria
- Conclusions:
- oral LD50 (rat) = 1815 mg/kg bw
- Executive summary:
10 rats per sex and dose were exposed to single doses of 2500, 3200, 4000, 5000, 6400 and 8000 cmm/kg test substance/kg via gavage. Directly after application accelerated breathing, tremors, apathy, abdominal position and clonic convulsions were observed. Within a 7 -day observation period, 2/20, 8/20, 19/20, 20/20, 20/20 and 20/20 animals died in the dose groups of 2500, 3200, 4000, 5000, 6400 and 8000 cmm/kg. Necropsy revealed venous congestion in deceased animals. A LD50 of 3300 cmm/kg was determined. The test material was an aqueous solution of the test subtance, containing 50% test substance. It is unclear whether the LD50 refers to the test material or pure test substance. Considering the test substance density, the LD50 of 3300 cmm/kg was converted to 3630 mg/kg. Applying a worst case approach, the LD50 was corrected to 1815 mg/kg bw accounting for the water content in the test material.
Reference
Table 1: Mortalities
Dose cmm/kg |
Concentration | Number of animals | Number of deceased animals within | |||
1 h | 24 h | 48 h | 7 d | |||
8000 | 30% | 10 M | 10/10 | 10/10 | 10/10 | 10/10 |
10 F | 10/10 | 10/10 | 10/10 | 10/10 | ||
6400 | 30% | 10 M | 10/10 | 10/10 | 10/10 | 10/10 |
10 F | 10/10 | 10/10 | 10/10 | 10/10 | ||
5000 | 30% | 10 M | 10/10 | 10/10 | 10/10 | 10/10 |
10 F | 10/10 | 10/10 | 10/10 | 10/10 | ||
4000 | 30% | 10 M | 9/10 | 9/10 | 9/10 | 9/10 |
10 F | 9/10 | 10/10 | 10/10 | 10/10 | ||
3200 | 30% | 10 M | 3/10 | 3/10 | 3/10 | 3/10 |
10 F | 5/10 | 5/10 | 5/10 | 5/10 | ||
2500 | 30% | 10 M | 0/10 | 0/10 | 0/10 | 0/10 |
10 F | 0/10 | 2/10 | 2/10 | 2/10 |
Endpoint conclusion
- Endpoint conclusion:
- adverse effect observed
- Dose descriptor:
- LD50
- Value:
- 1 815 mg/kg bw
Acute toxicity: via inhalation route
Link to relevant study records
- Endpoint:
- acute toxicity: inhalation
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Inhalation hazard test, saturated atmosphere was tested, acceptable for assessment
- Principles of method if other than guideline:
- Method: BASF test
- GLP compliance:
- no
- Remarks:
- GLP was not compulsatory at this time
- Test type:
- other: Inhalation hazard test
- Species:
- rat
- Strain:
- other: Gassner
- Sex:
- not specified
- Route of administration:
- inhalation: vapour
- Details on inhalation exposure:
- To generate saturated atmosphere, air was directed though a 5 cm layer of the test material at 20°C and 100°C respectively.
- Duration of exposure:
- 8 h
- No. of animals per sex per dose:
- 20°C vapour: 12 animals
100°C vapour: 12 animals - Control animals:
- no
- Dose descriptor:
- other: LT0
- Remarks:
- 20°C vapour
- Effect level:
- 13.75 mg/L air
- Exp. duration:
- 8 h
- Remarks on result:
- other: no mortality observed
- Dose descriptor:
- other: LT0
- Remarks:
- 100°C vapour
- Effect level:
- 43.36 mg/L air
- Exp. duration:
- 8 h
- Remarks on result:
- other: no mortality observed
- Mortality:
- No mortalities after 8-hour exposure to an atmosphere enriched with the test substance vapour generated at 20 and 100°C.
- Clinical signs:
- other: Irritation of mucous membranes was observed.
- Gross pathology:
- No adverse effects observed.
Reference
Endpoint conclusion
- Endpoint conclusion:
- no adverse effect observed
- Dose descriptor:
- discriminating conc.
- Value:
- 43 360 mg/m³
Acute toxicity: via dermal route
Endpoint conclusion
- Endpoint conclusion:
- no study available
Additional information
Acute oral toxicity
Three acute oral toxicity studies are available.
In the first study (1972), 10 rats per sex and dose were exposed to single doses of 2500, 3200, 4000, 5000, 6400 and 8000 cmm/kg test substance/kg via gavage. Directly after application accelerated breathing, tremors, apathy, abdominal position and clonic convulsions were observed. Within a 7 -day observation period, 2/20, 8/20, 19/20, 20/20, 20/20 and 20/20 animals died in the dose groups of 2500, 3200, 4000, 5000, 6400 and 8000 cmm/kg. Necropsy revealed venous congestion in deceased animals. A LD50 of 3300 cmm/kg was determined. The test material was an aqueous solution of the test subtance, containing 50% test substance. It is unclear whether the LD50 refers to the test material or pure test substance. Considering the test substance density, the LD50 of 3300 cmm/kg was converted to 3630 mg/kg. Applying a worst case approach, the LD50 was corrected to 1815 mg/kg bw accounting for the water content in the test material.
In the second study, six doses of the test substance were applied to 5-10 rats per sex and dose via gavage (1974). Dyspnea, abdominal position, convulsions, tremors, inbalance, mild atonia were obsrved at 3200 µL/kg and above. No adverse clinical effects were observed up to 1600 µl/kg. Within an 7d observation period, 0/10, 0/20, 6/20, 8/20, 8/10 and 20/20 animals died in the dose groups of 200, 1600, 3200, 4000, 5000 and 6400 µL/kg. Necropsy revealed venous congestion and dilatation of the heart in deceased animals. A LD50 of 4010 µL/kg was determined. The test material was an aqueous solution of the test subtance, containing 50% test substance. It is unclear whether the LD50 refers to the test material or pure test substance.
In the third study (1981), 5 rats per sex and dose were exposed to single doses of 681, 1000, 1470 or 2150 mg test substance/kg via gavage. Mortality and clinical symptoms were recorded within an 14 -day observation period. Deceased and surviving rats were subjected to necropsy. Animals showed dyspnea, apathy, prone position, tumbling, tremors, tonic-clonic convulsions, poor general condition, shaggy hair, alopecia and exsiccose. At 1470 mg/kg and above all animals died within one day. At 1000 mg/kg 2/5 animals died per sex during the course of the study. No animals died in the lowest dose group. Deceased animals showed venous congestion of the liver and gut atonia with diarrhetic content. No adverse effects were seen in the organs of killed animals. A LD50 of ca. 1000 mg/kg was determined. Since the test material contained other substances in concentrations >5%, this study was disregarded for assessment, because the toxicity could not be clearly attributed to the test substance.
Acute inhalation toxicity
Two hazard inhalation hazard tests have been perfomed, in which vapour of the test substance was administered to rats. To generate saturated atmosphere, air was directed though a 5 cm layer of the test material at 20°C or 100°C, respectively.
In the first test (1972), 12 rats (males and females) were exposed for 8 hours to vapors generated at 20°C or 100°C, equivalent to test substance concentrations of 13.75 and 43.36 mg/L, respectively. No mortality occured during the 8-hour exposure and the 7-day post-exposure observation period but irritation of mucous membranes was observed. No adverse substance-related effects were identified in the course of necropsy.
In the second test (1974), 12 rats (males and females) were exposed for 8 hours to vapors generated at 20°C, equivalent to a test substance concentration of 11.07 mg/L. In line with the results of the first test, no mortality or severe symptoms of toxicity occured during the 8 hour exposure and the 7 -day post-exposure observation period. Only a mild irritation of mocous membranes was observed. Necropsy revealed no adverse substance-related effects.
Acute dermal toxicity
No information available.
Justification for classification or non-classification
Classification, Labelling, and Packaging Regulation (EC) No. 1272/2008
The available experimental test data are reliable and suitable for classification purposes under Regulation 1272/2008. As a result, the substance is classified for acute oral toxicity Cat. 4 under Regulation (EC) No. 1272/2008, as amended for the thirteenth time in Regulation (EC) No. 2018/1480.
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