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EC number: 701-281-9
CAS number: -
Respiration rate and inhibition of samples
linear range (minutes)
mg O2/ Lat begin
mg O2/ Lat end
respiration rate(mg O2. L-1. h-1)
neg. control 1
test substance 1
test substance 2
test substance 3
test substance 4
test substance 5
neg. control 2
The study was performed to estimate possible effects of
"3-HYDROXY-2-(HYDROXYMETHYL)-2-METHYLPROPIONALDEHYDE" on aerobic
microbial sewage treatment plants. The test was performed according to
the OECD Guideline for Testing of Chemicals 209 "Activated Sludge,
Respiration Inhibition Test" and the EU-COUNCIL REGULATION (EC) No
440/2008, using activated sludge from a sewage treatment plant treating
predominantly domestic sewage.
Five concentrations of the test substance (7.7, 19.3, 48.2, 120.5 and
301.2 mg/L) were
tested versus two negative controls (tap water).
As positive control substance 3,5-dichlorophenol was used and tested in
concentrations (5.0, 12.1 and 29.8 mg/L).
The microbial inoculum was a preparation of activated sludge collected
on the day before the
test. 50 mL of synthetic sewage concentrate were added per litre and the
sludge was kept
areated. On the day of the experiment, the concentration of the
microbial inoculum was
adjusted to 4 g of dry weight per litre which results in a final
concentration of 1.6 g dry weight
per litre in the samples.
After mixing the inoculum with synthetic sewage solution and appropriate
dilutions of control
or test substance solutions the samples were aerated for a contact time
of 3 hours at 19 °C.
Negative controls were run before the first and after the last test
substance sample. After
incubation the respiration rates were determined in closed bottles using
an oxygen sensitive
electrode. The inhibition of respiration was calculated from the
respiration rates using the
mean value of the negative controls as 100 %.
EC10: greater than 300 mg/L
EC20: greater than 300 mg/L
EC50: greater than 300 mg/L
EC80: greater than 300 mg/L
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