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EC number: 701-281-9
CAS number: -
Validity of the test:
The test is considered valid if the
difference of extremes of replicate values of the removal of the test
substance at the plateau, at the end of the test or at the 10-d window,
as appropriate, is less than 20 % and if the percentage degradation of
the reference compound has reached the pass levels by day 14.
These criteria were fulfilled.
centrifugation of the day 17 samples the vessels of the individual
samples A33 and TK36 broke. Therefore no DOC analysis of these samples
could be performed.
Aim and methods
biodegradability of the test substance was determined by the measurement
of DOC (dissolved organic carbon) at frequent intervals over a 28 d
period. The method used was that described in Part C.4-B of the
Regulation (EC) 440/2008 and in the OECD Guideline 301 E.
reference substance sodium benzoate was degraded by 96.0 % within 14
in the toxicity control, which contained sodium benzoate and the test
substance, was not indicative of an inhibition of the microbial activity
by the test substance.
test substance with a nominal starting concentration of 20
mg DOC/L was not degraded within 28 days of incubation at a
mean temperature of 20.6 °C.
According to the
Guidelines a substance giving a result greater than 70 % loss of DOC in
a 10-d window within 28 days is regarded as "readily biodegradable".
According to the
results of this study, the test substance can therefore not be
classified as readily biodegradable.
ready biodegradability of the test substance was determined according to
OECD 301 E (1992). The
test substance was degraded 11.2 % within 28 days of incubation. The
test substance is considered to be non- biodegradable.
ready biodegradability of the test substance was determined by the
measurement of DOC (dissolved organic carbon) at frequent intervals over
a 28 days period according to OECD 301 E (1992).
test substance, which provided the sole source of carbon and energy, was
dissolved in buffered mineral salts medium at a nominal concentration of
about 20 mg organic carbon per litre. The medium wasinoculated with
micro-organisms derived from a sample of a sewage effluent from a
domestic waste water treatment plant not previously exposed to the test
substance. The vessels were incubated in darkness within a specified
temperature range for 28 days. The vessels were inserted in a shaking
machine and kept there until the end of the study. DOC-determinations
were performedin intervals. The
test substance was degraded 11.2 % within 28 days of incubation at a
mean temperature of 20.6 °C. The test substance is considered to be non-
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