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EC number: 218-235-4
CAS number: 2090-05-3
A known volume of (pretreated) anaerobic
sludge is suspended in an oxygen free medium and placed in a suitable
vessel leaving sufficient headspace into which any gases produced may be
evolved. The concentration of solids corresponds to about 10 % of that
usually present in a full scale digester. A small amount of test
substance of known carbon content is added to each of the test vessels
which are then sealed and incubated at a constant temperature and pH for
a period of up to 8 weeks. Any carbon dioxide and methane produced by
the inoculum is quantified from measurements on control digesters.
The biodegration rate of the test substance is >= 89 % over 21-35 days
under the conditions of this test.
Several non-guideline tests are available that investigate the biodegradability of the test substance under aerobic conditions. The outcome of the tests is indicative for rapid biodegradation in fresh water. Kohei (1985) finds 61-69% degradation (as BOD or DOC) over a period of 80 hours, MITI (1979) reported 98% degradation (as TOC) over 14 days, Howard (1981) >94.5% (decrease of radioactivity) over 7 days and Freitag (1985) 65.4% (CO2 evolution) over 5 days at a concentration of 0.05 mg/L test substance.
In seawater the test substance degrades 10-80% (DOC removal) over a period of 8-28 days (Shimp 1987), but the test is performed without adding a specific inoculum to the water. Two tests with similarities to simulation testing are available (Junker 2009) that indicate that in a water sediment system the test substance degrades > 74.4% over a 8-day period. These studies are considered to be the key studies.
Several studies indicating that the test substance is completely biodegradable under anaerobic conditions are available (Birch, 1989; Uma, 2007;Nottingham, 1969; Kameya, 1995). The key study for anaerobic degradation (Birch, 1989) is performed according to the methods as described under OECD 311.
Based on the large number of studies indicating a rapid and almost
complete biodegradation, it can be concluded that the test substance is
readily biodegradable. Although most tests do not provide information on
the passing of the 10-day window, this is considered to be passed,
because the overall test duration is in most tests shorter than 10 days.
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